Hello All,
I'm delighted to announce that Dr. Allan D'Arcy will give a scientific presentation in Rigaku Crystallization Webinar on September 4th, 2013, 8:00am Pacific Daylight Time. The webinar is free to attend and all are welcome. You can register it through our web page, http://www.rigakuautomation.com/webinars. Here is the detail of the presentation: " Nucleation and seeding in protein crystallization Presenter: Dr. Allan D'Arcy Date and time: Wednesday, September 4th, 2013, 8:00am Pacific Daylight Time The nucleation event in protein crystallization is a part of the process that is poorly controlled. It is generally accepted that the protein should be in the metastable phase for crystal growth, but for nucleation higher levels of saturation are needed. Formation of nuclei in bulk solvent requires interaction of protein molecules until a critical size of aggregate is created. In many crystallization experiments sufficiently high levels of saturation are not reached to allow this critical nucleation event to occur. If an environment can be created that favors a higher local concentration of macromolecules, the energy barrier for nucleation may be lowered. When seeds are introduced at lower levels of saturation in a crystallization experiment, nucleation may be facilitated and crystal growth initiated. The different techniques used for seeding such as streak, macro and seed bead will be described and particular emphasis will be given to the method of automated microseed matrix seeding which has proved to be an extremely powerful tool for obtaining and optimizing hits. 1. D'Arcy A, Villard F, Marsh M: An automated microseed matrix-screening method for protein crystallization. Acta Crystallographica Section D-Biological Crystallography 2007, 63:550-554. 2. Ireton GC, Stoddard BL: Microseed matrix screening to improve crystals of yeast cytosine deaminase. Acta Crystallographica Section D-Biological Crystallography 2004, 60:601-605 3. D'Arcy A, Mac Sweeney A, Haber A: Using natural seeding material to generate nucleation in protein crystallization experiments. Acta Crystallographica Section D-Biological Crystallography 2003, 59:1343-1346." Thank you, Jian Xu, Ph.D. Director of Scientific Marketing Rigaku Automation, Inc. jian...@rigaku.com<mailto:jian...@rigaku.com> www.rigakuautomation.com<http://www.rigakuautomation.com>
