Re: [ccp4bb] SARS-CoV-2 test on a smartphone

James, you know you can generate heat within the LAMP test, there’s chemistry for that and very accurate, sufficient for 30 - 45 minutes “runtime" But I enjoy reading your emails. Jürgen > On Dec 7, 2020, at 8:50 PM, James Holton wrote: > > > Yes, I would like to congratulate all these

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

Yes, I would like to congratulate all these authors for getting this out. Many of them are friends and colleagues, and it is great to see them doing well, and getting this kind of work in front of as many eyes as possible.  The nice thing about this particular assay is that it is linear with

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

Hi everyone, I wanted to revive this discussion with a fresh paper from Cell journal: https://www.cell.com/cell/fulltext/S0092-8674(20)31623-8 So one could use a smartphone camera for SARS-CoV-2 detection but you still need some extra tools, like 488 nm laser. пт, 10 апр. 2020 г. в 20:14, James

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

It looks to me that in this norovirus test the phone is acting as nothing more than a camara attached to a conventional microscope. Light source is 3rd party, and the microscope body is 3D printed. 3D printing is cool and all, but it does not scale well.  Antibodies are also expensive to

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

Here’s another link I found that should make this project feasible: https://physicsworld.com/a/smartphone-based-device-detects-norovirus/ Jürgen > On Apr 2, 2020, at 3:52 PM, Patrick Shaw Stewart > wrote: > > Jurgen,

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

Jurgen, that *was *interesting. (Strange how your hair came and went during the talk, leaving you bald sometimes - but of course that didn't matter ! ;) Did you know that coronavirus was first isolated at 33C and that this temperature may be required for isolation?

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

To be honest I would set up reasonably equipped test centers - yes I realize that sample shipping is a drag - but probably still cheaper than in other countries. There are foundations with good budgets for this and the machinery is much more affordable these days. Again there would be no need for

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

Hi Artem, How would you do this in resource limited countries? I think James idea is pointing toward that direction, deliver an easy to apply reagent, take your phone and detect. It still is challenging in most African countries but more doable, there are more smartphones e.g. older Android &

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

For DIY fanatics out there: Step 1. Express reverse polymerase (single subunit one for simplicity, like the one from swine retrovirus). For hardcore fans also express Taq. That is easy. Purify both with minimal RNAse contamination. Less easy but we know how to do that. Step 2. Design and order a

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

I’m sharing a laymen’s talk I recently gave on some aspects of Corona. I’m not claiming to be an expert, but there is useful information in the presentation. I skipped the intro and zoomed directly to the start of my presentation. https://www.youtube.com/watch?v=B00tJnbktVo=youtu.be=204

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

Personally, if I were infected with SARS-CoV-1 instead of SARS-CoV-2 I'd still like to know that. It is most certainly true that the primer design must be done right: checking for self-annealing, low genomic variability, cross-reactivity to potential contaminants etc.  Fortunately, we have

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

Agreed! False-positives would be less likely in this pandemic situation, although I am not sure about the predominance of - and similarity with - other common cold-causing coronaviruses. Regards, Sahil Batra On Thu, Apr 2, 2020 at 9:03 AM Nagarajan V wrote: > This is only a theoretical

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

This is only a theoretical distinction, isn’t it, given that it’s CoV2 that is predominant right now? V. Nagarajan On Tue, Mar 31, 2020 at 11:56 PM Sahil Batra wrote: > Dear Prof. Holton, > > An innovative idea; however all of the 30 kb genome may not be useful for > specific detection -

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

Dear Prof. Holton, An innovative idea; however all of the 30 kb genome may not be useful for specific detection - SARS-CoV1 and SARS-CoV2 share 80% identity. A similar fluorescent detection approach for SARS Cov2 -- using the indiscriminate collateral activity of Cas12 nuclease -- has been

Re: [ccp4bb] SARS-CoV-2 test on a smartphone

One problem I see is the sputum, there’s a reason why swabs are made to get sufficient viral material. Since stool samples test PCR positive that might be an easier approach to get sufficient viral material. As a side note, these are not infectious anymore, or at least one has not been able to

[ccp4bb] SARS-CoV-2 test on a smartphone

In order to do global survelinace of this new virus I figure we're going to need billions of tests.  The biggest barriers I believe are logistical.  Shipping back and forth to a central labs isn't going to cut it, and neither are test kits that cost $800 each. I think I may have a plausible