Email: esn...@hwi.buffalo.edu
[cid:image003.png@01D2D241.8F507E50]
Heisenberg was probably here!
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hena Dutta
Sent: Saturday, May 20, 2017 5:15 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] crystallization screen for protein
Hena
There was a very interesting paper by Peter Sun and coworker from 2002.
They pointed out that there is a very strong bias towards crystallizing
protein-protein complexes with PEG rather than salt as the main precipitant.
Patrick
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Radaev and Sun.
Dear Members,
I am trying to crystallize a protein-protein complex. Both are soluble
proteins and form complex as observed in FPLC either co-purifying or
separately purifying and then mixing with equi-molar ratio.
Looking for suggested screens to try for crystallization.
So far tried with Qiagen
