Dear Dhiraj,
You can use coiled-coil domain to dimerize your protein. Please look at those
exemplary publications:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923517/#R12
https://www.nature.com/articles/nsb877
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375751/
Dear Colleagues,
With regard to the use of GST as a dimerizing fusion partner, the
following paper from my lab provides a well documented example:
https://pubmed.ncbi.nlm.nih.gov/9336840
Cheers, Arne
Am 24.09.20 um 09:27 schrieb Barone, Matthias:
Dear Dhiraj
I just recently had to check
Hi Dhiraj,
you could also consider making a fusion protein of your protein with itself,
with a suitable long linker (gly-ser-gly-ser etc.?) in between. At least that
dimer won't dissociate.
Best,
Herman
Von: CCP4 bulletin board Im Auftrag von Srivastava,
Dhiraj
Gesendet: Mittwoch, 23.
Thanks everyone for all the nice suggestions.
regarding Matthew's question, Yes, There is a possibility of polydispersity due
to the proteins making chain/aggregate, however the affinity between the
dimeric protein A and monomeric protein B is poor and kinetics of dissociation
is very fast thus
I was thinking the form of GFP that dimerizes. This would also make it
easy to track where the protein is.
On Tue, Sep 22, 2020 at 1:28 PM Diana Tomchick <
diana.tomch...@utsouthwestern.edu> wrote:
> Any dimeric tag should work if you add a long enough linker to satisfy
> your distance
Hi Dhiraj,
You could try a C-terminal Fc-tag if it is an extracellular protein,
e.g. see pHL-FcHis vector described in the supplement of PMID
17001101.
Best wishes,
Tomas
On Tue, Sep 22, 2020 at 6:08 PM Srivastava, Dhiraj
wrote:
>
> Hi
> I want to make my protein dimeric to increase its
Any dimeric tag should work if you add a long enough linker to satisfy your
distance criterion.
GST, for example. Download the coordinates and get a rough idea how long the
linker would have to be for your protein.
Diana
**
Diana R. Tomchick
Hi
I want to make my protein dimeric to increase its affinity for its
interaction partner which is a dimer. does anyone know a suitable tag/fusion
protein which can be used as C terminal fusion for this purpose? I can not use
any of the leucine zipper as I am looking for the distance
