] On Behalf Of Artem
Evdokimov
Sent: Tuesday, January 13, 2009 8:10 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] offtopic__which crystals to harvest
Hi,
A few simple hints:
(Please note that I am aware of the inexact language in the statements below
but I don't have the time to write
of V. Nagarajan
Sent: Wed 1/14/2009 2:49 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] offtopic__which crystals to harvest
Actually, UV fluorescence imaging appears to be a pretty reliable means of
discriminating protein crystals, provided your protein has Trp residue(s).
V. Nagarajan
JAN
Hi there,
Since a lot of different forms of crystals shows, I am using a quick/simple
strategy to choose crystals by applying a force on the crystal against the
wall, with the nylon loop.
Some can never break apart, so they are salt crystals? The others can not
survive the force and lose
various quasi-crystalline artefacts.
Good luck,
Artem
_
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
deliang
Sent: Tuesday, January 13, 2009 10:50 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] offtopic__which crystals to harvest
Hi there,
Since a lot
1. Pick many representative samples from each morphological class.
2. Take as many notes about the appearance, habits, and physical
properties of each crystal that you can.
3. Screen every crystal for diffraction in a variety of cryo-
protectants, etc.
4. All protein crystals were meant to be
