Hi Firdous,
For salt conditions try malonate:
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2356-8. Epub 2003 Nov
27.
Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown
macromolecular crystals.
Holyoak T1, Fenn TD, Wilson MA, Moulin AG, Ringe D, Petsko GA.
Dear Firdous,
Probably other people already suggested above, and I don't know which kind
of protein in your case. Here is my suggestion, you may try sugar solution
or polysaccharide solution as cryoprotectant. The whole idea is to provide
alternative H-bond network to support protein freezing.
Hi Firdous,
Dry mineral oil usually works well, and it does not alter the
crystallisation drop composition. Even better if you are using sitting
drop. Just add enough mineral oil to cover the crystallisation drop, and
you even have a lot more time to pull your crystal out before the high
t: [EXTERNAL] [ccp4bb] suggestions for cryoprotectant
Dear members
I have got beautiful crystal hits in SaltRx screens which are not diffracting
to a good resoultion. All of them are salt based condition and I am not able to
formulate a good cryoprotectant for these crystals. I also think that
Condition #3 isn’t far from the saturated conditions for lithium sulfate.
Perhaps slightly increasing the lithium sulfate concentration will work, or
using a related lithium salt.
See
Cryosalts: suppression of ice formation in macromolecular crystallography
Acta Cryst D
Volume
for cryopreservation.
Good luck.
Regards,
Vaheh Oganesyan, PhD
www.medimmune.com
From: CCP4 bulletin board On Behalf Of Firdous Tarique
Sent: Friday, October 19, 2018 5:58 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] suggestions for cryoprotectant
Dear members
I have got beautiful crystal
his e-mail
From: CCP4 bulletin board On Behalf Of Firdous Tarique
Sent: 19 October 2018 22:58
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] suggestions for cryoprotectant
Dear members
I have got beautiful crystal hits in SaltRx screens which are not diffracting
to a good resoultion. All of the
2018 2:08:18 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] suggestions for cryoprotectant
Dear Friduous,
This is also a good paper to look at once you know your crystals diffract at
room temperature:
https://www.ncbi.nlm.nih.gov/pubmed/10944336
Many of the salts in your condition will b
Dear Friduous,
This is also a good paper to look at once you know your crystals diffract at
room temperature:
https://www.ncbi.nlm.nih.gov/pubmed/10944336
Many of the salts in your condition will be cryo-protective if you increase
their concentration to saturated levels.
Cheers,
Joseph.
This is the first thing you need to do before going down the rabbit hole
looking for a cryo protectant. Mitegen make it much easier then in the 'old
days' of capillaries.
Good luck,
Len
lmtho...@ou.edu
On Oct 20, 2018 2:02 AM, Andreas Förster wrote:
Dear Firdous,
get some Mitegen sleeves
Hi,
have a look at the following interesting papers
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466044/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852300/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252874/
Good luck
J.
Am 19.10.18 um 23:57 schrieb Firdous Tarique:
Dear members
I
Dear Firdous,
get some Mitegen sleeves and test your crystals at RT. Before you establish
diffraction, there’s no need to agonize about cryo-conditions.
Good luck!
Andreas
On Fri, 19 Oct 2018 at 23:57, Firdous Tarique
wrote:
> Dear members
>
> I have got beautiful crystal hits in SaltRx
Dear Fridous
Have a look at https://www.ncbi.nlm.nih.gov/pubmed/14646118
Malonate as a cryoprotectant
Or https://www.ncbi.nlm.nih.gov/pubmed/20606259
Regards
Christine
On Fri, Oct 19, 2018 at 2:57 PM Firdous Tarique
wrote:
> Dear members
>
> I have got beautiful crystal hits in SaltRx
Hi Firdous,
I noticed that conditions 2,4 and 5 are overall harsh agents (chaotropic
agents such as KSCN (probably not KCN) and Nitrate ions ), in my own
personal experience I have never been very lucky with crystals grown in
nitrate ions.
Condition 1 however looks a bit more soft with 'gentle'
Dear members
I have got beautiful crystal hits in SaltRx screens which are not
diffracting to a good resoultion. All of them are salt based condition and
I am not able to formulate a good cryoprotectant for these crystals. I also
think that in my case the poor resolution is due to a poor
Dear All,
Since 80% saturated Li2SO4 has not been mentioned, I will do so. It is a
good cryosalt and I have often used
it even without any buffer added.
see:
Vera, L., Stura, E. A. (2013) Strategies for protein cryocrystallography.
Crystal Growth Design, e-print
Hello,
Does anybody know what would be a good cryoprotectant for the following
condition:
800 mM Sodium phosphate monobasic/1200 mM Potassium phosphate dibasic 100
mM Sodium acetate/Aceticacid pH4.5
Thanks
Deepak
Lots of choices. I usually try the crystallization solution + 30% glucose
first. Glycerol or ethylene glycol are other possibilities here. Another
possibility is 2.5-3.0 M sodium malonate at a similar pH.
Roger Rowlett
On Feb 6, 2014 11:40 PM, Deepak Thankappan Nair deepaktn...@gmail.com
wrote:
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Deepak
Thankappan Nair [deepaktn...@gmail.com]
Sent: Thursday, February 06, 2014 11:40 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] suggestions for cryoprotectant
Hello,
Does anybody know what would be a good cryoprotectant
Try L-proline. It works well with high ionic strength conditions:
http://www.ncbi.nlm.nih.gov/pubmed/22868767
Sent from Jack's iPad
On Feb 6, 2014, at 10:40 PM, Deepak Thankappan Nair
deepaktn...@gmail.commailto:deepaktn...@gmail.com wrote:
Hello,
Does anybody know what would be a good
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