Re: [ccp4bb] Lysine coordinated ions

2014-07-07 Thread Afshan Begum
Hi Kaherine, It seems its a modified lysine in to kcx (Carbamylated lysine) but its only the case if you used during entire purification or crystallization BME in your buffer Best Regards AFSHAN On Wednesday, July 2, 2014 5:38 PM, Katherine Sippel katherine.sip...@gmail.com wrote:

Re: [ccp4bb] Lysine coordinated ions

2014-07-01 Thread Keller, Jacob
… I manually attempted chlorine but the density said no. How did the density say no? Too much, too little…? I guess the bonds are too short anyway. What about anomalous signal using the awesomely-sensitive LLG maps from Phaser? Depending of course on resolution, Cl- can be quite visible, if

Re: [ccp4bb] Lysine coordinated ions

2014-07-01 Thread Nat Echols
On Tue, Jul 1, 2014 at 3:10 PM, Katherine Sippel katherine.sip...@gmail.com wrote: My google-fu has failed me once again so I am turning to the collective knowledge of the bb. I'm working on a blobology challenge at the moment and have hit a wall. Is anyone aware of an ion that coordinates to