Using mixed solvents is another approach:
Ciccone L., Tepshi L., Nencetti, S. Stura E.A. (2014)
Transthyretin complexes with curcumin and bromo-estradiol: Evaluation of
solubilizing multicomponent mixtures New Biotech. 32:54–64
http://dx.doi.org/10.1016/j.nbt.2014.09.002
Volatile solvents
Yes, this is tough. We mostly have used DMSO or DMF. You can try detergents,
but they tend not to be that effective in solubilisation and they might bind to
your protein rather than the compound you 'd like to bind. If you'd like to be
adventurous you could try using cyclodextrins as a
Monica,
I struggled with this a lot too. I had some success with low molecular
weight PEG (100-400) as a solvent, its well worth a try.
-bob
On Wed, Oct 15, 2014 at 9:13 AM, Monica Mittal monica.mitta...@gmail.com
wrote:
Dear All
Can anyone give suggestions for handling the solubility
Since you mentioned EtOH, why not do this:
-Make a tray with the appropriate mother liquors
-Make a drop for each well containing mother liquor and high-concentration
ligand in EtOH (you could vary the ratios here as needed.)
-Equilibrate by vapor diffusion until EtOH all goes into the well soln
An alternative is to dissolve your compound in MeOH and dispense it either
manually or via robot, let the plate sit sometime in the hood for faster
evaporation and then add your protein + reservoir.
Jürgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public
