[ccp4bb] PhD and postdoctoral positions open

Dear all I would appreciate if you could forward the included announcement to people you know that may be interested. With best regards, Raimond Ravelli ++ PHD STUDENTS AND POSTDOCTORAL POSITIONS IN STRUCTURAL BIOLOGY AT THE LEIDEN UNIVERSITY MEDICAL CENTER TWO PhD STUDENTS

[ccp4bb] X-Ray crystallisation scientist position at Evotec UK

Apology for this job advert coming from an unofficial email address. Evotec (UK) Ltd. wishes to recruit a scientist for a full time position supporting the X-ray Crystallography team. The position will be involved in providing services in crystallisation of proteins for drug discovery

[ccp4bb] Postdoctoral position – DNA repa ir - Paris

Postdoctoral position – Structural Biology of DNA repair A postdoctoral position is available to study DNA repair proteins involved in double-strand break repair. This post-doc is founded by Institut National du Cancer (INCa). All candidates must have a broad experience ranging from

[ccp4bb] pointless and HKL2000

Hi everyone, I would like to us pointless in CCP4 to check the laue group of a crystal. Since pointless needs unmerged data, my question is how to generate the unmerged data for pointless from HKL2000 ? Thanks, Simon

Re: [ccp4bb] pointless and HKL2000

just use the no merge original index command, which you can add during scaling using a macro. Just type no merge original index in the macro field during scaling. Then you'll have to import unmerged data to an mtz file using combat, if I remember correctly. hth, ciao sebastiano Monday, October

Re: [ccp4bb] pointless and HKL2000

Hi Simon, If you output no merge original index from scalepack, and (I assume) don't impose any symmetry on the data, so scale in P1, pointless will be able to work with the output. You will need a relatively recent version of pointless though, from ftp://ftp.mrc-lmb.cam.ac.uk/pub/pre Best,

Re: [ccp4bb] Protein-DNA complex prepartion for crystallization

Hi, Sadly, that happens sometimes. 1) make sure you have some salt in the DNA stock too 2) try (NH4)2SO4 instead of NaCl (just don't add Ca++, and remember the ionic strength of a given molarity will be higher) 3) if you have to, try more salt 3) try different ends on your oligos - sometimes

Re: [ccp4bb] refmac and average B factors

On Saturday 04 October 2008 18:32:00 Engin Ozkan wrote: Hi everyone, I was in the middle of creating a Table 1 for a finished structure and was puzzled by one number. It is the average B factor, especially in the case of TLS-refined structures. In this case, the average reported by

Re: [ccp4bb] Reading the old literature / truncate / refinement programs

Hi Ian, I did not follow up our recent debate about the respective merits of various truncate procedures, in particular the comparison of the French Wilson (1978) and Sivia David (1994) methods. Since you have extended the previous simulations, I feel that the discussion should be