The Protein Data Bank in Europe (PDBe) is seeking to recruit a
structural analyst/programmer to join the team at the European
Bioinformatics Institute located on the Wellcome Trust Genome Campus
near Cambridge in the UK.
For further information and to apply, please see the full EMBL-EBI job
Dear all,
I am posting an advertisement for a tenure-track group leader position for
structural biology on membrane proteins on behalf of Prof. Dr. Robert Tampé,
Institute of Biochemistry, Biocenter, Goethe-University Frankfurt.
Please reply to mailto:applicat...@biochem.uni-frankfurt.de
Well - we ought to be! There is an awful lot of unpredictable slog
goes on before we have the fun!
Eleanor
On 13 February 2014 12:50, Mike S mds...@gmail.com wrote:
I'm sorry, but did you just use the words crystallographers and modest
in the same sentence? :-)
On Thu, Feb 13, 2014 at
Let me pick up Eleanor’s comment:
is there something like a crystallographer today ? I mean in the true sense ?
I think as a “crystallographer” you won’t be able to survive the next decade,
you need to diversify your toolset of techniques as pointed out in this article
For some simulated phasing experiments, I want to create a lower resolution
diffraction data set by truncating a high resolution data set. I would like to
avoid Fourier ripples due to the truncation of the high resolution data by
downscaling the data such that I/sigma=2.0 in the highest
Dear CC4BBers,
I am trying to figure out what is the best way to determine the protein
concentration of my membrane protein. My purified membrane protein is in
20mM Tris pH 7, 150mM NaCl and 0.02% DDM (CMC of DDM=0.0076%).
After reading the friendly manuals and searching online, I've learned
Let me pick up Eleanor#8217;s comment:is there something like a
crystallographer today ?
Yes, James Holton and countless others.
I mean in the true sense ?I think as a #8220;crystallographer#8221; you
won#8217;t be able to survive the next decade, you need to diversify your
toolset of
One comment (not a complaint) on all this: it seems like the same questions
get asked over and over again. If there is a good place for a general
crystallography FAQ list it is well past time for one to be put together -
or maybe it just needs to be better advertised? At a minimum, for instance:
I think you are talking about the R of the reflections in an image to the set
of unique reflections resulting from scaling.
Here is a definition of Rmerge from a dtscaleaverage log file:
In the tables below Rmerge is defined as:
Rmerge = Sum Sum |Ihi - Ih| / Sum Sum Ih
h i
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On 02/13/2014 04:21 PM, Nat Echols wrote:
Some of the other common queries (name my blob!) still need to be
handled on a case-by-case basis, but it would be much more
efficient for everyone if the standard answers were collected
somewhere
Nat - two such places already exist! They are the official ccp4 wiki at
http://ccp4wiki.org/ and the unofficial one (but a very useful one!) at
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/
Eugene
On 13 February 2014 15:21, Nat Echols nathaniel.ech...@gmail.com wrote:
One comment (not a
I guess somebody could add it here perhaps ?
http://ccp4wiki.org/~ccp4wiki/wiki/index.php?title=Special:Allpages
or start their own wiki on Tips tricks in crystallography and why you
should always have olive oil when traveling to a synchrotron (well nowadays
it’s becoming more difficult to
By applying a high temperature factor, one should not suffer Fourier ripples,
since the missing high resolution reflections have negligible intensities, or
put differently, one simulates a poorly diffracting crystal.
Best,
Herman
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board
Hi Folks,
Like many other folks who read, benefit and hopefully contribute to this
bulletin board, I am required to stay abreast of the latest cloning,
heterologous membrane protein expression,
purification, biochemistry, crystallization, and structure solution
techniques, as a matter of sheer
The CCP4bb is great.. it truly is.
The access to experts and their experience (probably the most valuable) is
unparalleled.
However, mailing lists to organize discussions and disseminate new ideas is
just so ... 90s.
Wikis? maybe you've just crossed into the new millenium.
These days, if
Roger,
While I agree with your list, the BCA assay does not use molybdate (as we make
it from scratch with bicinchoninic acid, sodium carbonate, sodium bicarbonate,
sodium tartrate, and cupric sulfate pentahydrate). For membrane proteins, I
prefer the BCA assay until the protein is pure
Dear Francis,
could you elaborate how something like Quora (what is it) would save you
time compared to en email based? From what I gathered at wikipedia,
Quora is web based, and in my experience web based services usually
follow Wirth's law: they are slow.
Cheers,
Tim
On 02/13/2014 05:47
Hi Everyone,
Thanks very much for your helpful responses and suggestions. I will use the
BCA assay.
Cheers,
Raji
On Thu, Feb 13, 2014 at 11:27 AM, R. M. Garavito rmgarav...@gmail.comwrote:
Roger,
While I agree with your list, the BCA assay does not use molybdate (as we
make it from
Hi everyone
Can anyone please tell me the three letter code for D-Ribulose
1,5-bisphosphate..
Thanks in advance
--
Regards
Faisal
School of Life Sciences
JNU
It is RUB.
http://xray.bmc.uu.se/hicup/RUB/index.html
On Thu, Feb 13, 2014 at 1:07 PM, Faisal Tarique faisaltari...@gmail.comwrote:
Hi everyone
Can anyone please tell me the three letter code for D-Ribulose
1,5-bisphosphate..
Thanks in advance
--
Regards
Faisal
School of Life
Hi Bingfa,
first suggestion would be to process your data with the anomalous flag turned
on - just in case you happen to have some metal bound coincidentally and you
happen to have collected at a decent wavelength to pickup some anomalous
scattering. ~30% of proteins in the PDB have a metal
All, I'm having problems refining a structure with an N-terminal acetylated
MET residue. I'm trying it with both Refmac Buster. Buster works fine
gives perfect planar geometry for the ACE-MET linkage. Refmac gives a
pyramidal acetyl group after refinement which to my eyes is wrong (sp2 C
On Feb 13, 2014, at 4:53 PM, Sun Bingfa sunbin...@gmail.com wrote:
Now we have dataset to ~4.2 Angstrom and using extracellular homolog
structure we can find a solution for this part(~45% of the whole molecule MW)
through molecular replacement, and the molecules are packed as layers, and
Dear all,
I am looking for a postbac for a membrane protein structure-function
project in my lab at the NIH. The NIH Postbac IRTA program provides
recent college graduates who are planning to apply to graduate or
professional (medical/dental/pharmacy) school an opportunity to spend
one or two
Hi Raji,
There are also some proprietary stains such as the 660 nm (can't
they think of a better product name?) stain from Pierce that are detergent
compatible. I used this briefly with success when comparing against Abs 280
nm.
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant
Dear All,
May be a stupid question. But if we take buffer with detergent as control
(Blank), would not the difference in ODs using any of the methods used e.g.
Bradford assay, gives protein concentration?
Regards
Nishant
On Thu, Feb 13, 2014 at 9:09 PM, Roger Rowlett rrowl...@colgate.edu wrote:
Nat,
that's why I set up the CCP4 wiki at
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Main_Page ! The
idea is that everybody benefits: experienced crystallographers/biologists can
concentrate on the new and difficult questions coming up on the bulletin board,
and novices find
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