Is there any opinion or experience about whether Lion is ready for
crystallographic use? Should I upgrade?
Phil
Phil,
On Fri, Sep 9, 2011 at 4:28 AM, Phil Evans p...@mrc-lmb.cam.ac.uk wrote:
Is there any opinion or experience about whether Lion is ready for
crystallographic use? Should I upgrade?
MacPyMOL works fine on Lion.
Cheers,
-- Jason
--
Jason Vertrees, PhD
PyMOL Product Manager
Schrodinger,
Dear Crystallographers,
I have set hanging drop trays with 2ul of protein and 2 ul of resorvior
solution. I have seen in some cases the drops are swelling. My protein
buffer has 15% glycerol in it.
This is happening mainly when I have peg 400 or peg MME or MPD or Jeffamine
in the buffer condition.
Yes - add glycerol to the reservoir -
15% glycerol has a huge influence on the vapour diffusion gradients
Poul
On 09/09/2011, at 13.22, anita p crystals...@gmail.com wrote:
Dear Crystallographers,
I have set hanging drop trays with 2ul of protein and 2 ul of resorvior
solution. I have
The following is simply a quote from a recent post by Enrico Stura.
Probably has a lot to do with what you are finding:
Glycerol is also great to reduce nucleation. If you decide to add
glycerol to the protein solution (for solubility, but in your case it
might be for stability
reasons), you
Dear Anita
You are seeing the effects of a very modest reduction of vapor pressure of
water with polymers. You could add salt to your reservoir and you could insure
your drops are at the same temperature as your reservoir - not easy in
conventional constant temp incubators.
George
Sent via
Anita,
see the message I posted yesterday on the CCP4bb:
http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg22638.html
or google stura glycerol
Re: [ccp4bb] How to help crystal grow bigger
In your case the sentence you need is:
You need to have a higher (double) glycerol concentration in the
Hi Everyone,
Sorry for the naive and non-CCP4 question.
Is it possible to precipitate proteins (TCA, acetone) from a sample
that has already been stored in protein loading dye? The protein is
too dilute in my current sample and I basically want to load all of
the sample (100uL) in a single well
Are you sure that 100 uL won't fit in your
loading well? Commercial 10x10 cm minigels have quite large
loading wells. I think it's possible to squeeze as much as 50 uL
into a standard 10-well, 1 mm gel. If you are using typical
discontinuous SDS-PAGE with a stacking
Structural studies on ribosomal RNA modification enzymes:
A postdoctoral position in X-ray crystallography is available immediately in
the group of Dr. Graeme L. Conn, in the Department of Biochemistry at Emory
University School of Medicine in Atlanta GA, USA. This opening is part of a
Is it possible to precipitate proteins (TCA, acetone) from a sample
that has already been stored in protein loading dye? The protein is
too dilute in my current sample and I basically want to load all of
the sample (100uL) in a single well in the gel. Unfortunately, I
already added protein dye
Is it ok for you to heat your sample? If so, you could near-boil your
sample in a heating block, say at 95 degrees C, in order to concentrate
it. I've done this in regular 1.5 mL eppendorf tubes with either the cap
open or where I've punctured the top of the cap with a narrow bore needle
(in order
Hi SnowDeer-
What's your precipitant? Have you tried lowering that as much as possible?
This will likely delay nucleation and crystal growth but the crystals will
also likely be larger (and perhaps more well ordered). Of course, you may
reach a lower limit where you prohibit nucleation. You'll
Dear CCP4'ers
In the input instructions for ALMN it says:FIND peak maxpek [RMS] [OUTPUT
filename]
Read peak threshold and maximum number of peaks. MAXPEK is the maximum number
of peaks to find (default = 20). Up to MAXPEK peaks above PEAK will be found,
and all symmetry related peaks
On Thu, 2011-09-08 at 16:58 +0200, Miguel Ortiz Lombardía wrote:
Perhaps the Nd/DN issue was solved between revisions 3470 and 3628 ?
Indeed. I just built the rev 3633 and it works fine. Autobuild scripts
used to have some problems on Lucid, but this time it worked perfectly.
This evolved
Hi Rex
I would assume it's the RMS deviation of the rotation function value from
the mean. If so then it's defined in the same way as the population
standard deviation as given here:
http://en.wikipedia.org/wiki/Standard_deviation
Cheers
-- Ian
On Fri, Sep 9, 2011 at 4:20 PM, REX PALMER
Rex, sorry, addendum to my last post: the grid points in the Eulerian space
of the cross-RF (which is what I assume you're talking about) do not occupy
equal volumes in angular space as they would do in a Euclidean space (e.g. a
normal electron density map). For example the volume of any grid
Hi Phil:
I've found few, if any advantages. I fear for the future.
I've had problems getting coot to run stereo due to the X11 implementation in
10.7. Apart from that, no major problems with crystallographic software.
Lion greedily uses memory, and any computer I have with less than 4 gig of
I have noticed, in new versions of OSes, that there generally is
rampant violation of the concept of if it ain't broken, don't fix.
Shouldn't there be more moments of delight, when you see they have
solved a previous poorly-engineered feature with an elegant solution?
But a lot of the time, you
On Sep 9, 2011, at 11:09 AM, William Scott wrote:
(nothing us usual in of itself)
I forgot to mention how delightful the spelling auto-correction feature can
be. (It should have read nothing unusual in and of itself).
That, at least, can be turned off.
Peking University, College of Life Sciences seeks to recruit dedicated
postdoctoral fellows to carry out structural and functional investigation
of cell surface receptors in nervous systems.
The successful candidates will focus his/her research on elucidation of
molecular mechanism with which
Still doesn't beat my all-time favorite, an early Microsoft spell-checker that
changed diffract to defrocked.
I forgot to mention how delightful the spelling auto-correction feature
can be. (It should have read nothing unusual in and of itself).
That, at least, can be turned off.
Shoshana Wodak once told me that Word kept suggesting
Shoeshine Kodak as the correct spelling of her name.
I just tried my copy of Word and it seems to have improved.
Frances
=
Bernstein + Sons
* *
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