Adding Tacsimate on top of the basic conditions worked best for us.
On Tue, Dec 23, 2014 at 11:11 AM, rohit kumar rohit...@gmail.com wrote:
Dear all,
I got crystals in 1.4 M sodium/potassium phosphate, ph-8.2 condition.
Could anyone tell me what is the best cryprotectant for this above
You can try adding 10% or 20% glycerol to your Cryo that works for us.
On Tue, 23/12/14, Yarden Opatowsky yarden.opatow...@gmail.com wrote:
Subject: Re: [ccp4bb] best cryprotectant
To: CCP4BB@JISCMAIL.AC.UK
Date: Tuesday, 23 December, 2014, 2:47 PM
Ansuman,
you can calculate arbitrary merging stats, including CC1/2, Rmeas from
*unmerged* intensities, with a program like AIMLESS
http://www.mrc-lmb.cam.ac.uk/harry/pre/aimless.html#data_from_denzo. By
default, HKL2000 (scalepack) will have output *merged* intensities, but
merging is inherently