Re: [ccp4bb] best cryprotectant

2014-12-23 Thread Yarden Opatowsky
Adding Tacsimate on top of the basic conditions worked best for us. On Tue, Dec 23, 2014 at 11:11 AM, rohit kumar rohit...@gmail.com wrote: Dear all, I got crystals in 1.4 M sodium/potassium phosphate, ph-8.2 condition. Could anyone tell me what is the best cryprotectant for this above

Re: [ccp4bb] best cryprotectant

2014-12-23 Thread tarun arya
You can try adding 10% or 20% glycerol to your Cryo that works for us. On Tue, 23/12/14, Yarden Opatowsky yarden.opatow...@gmail.com wrote: Subject: Re: [ccp4bb] best cryprotectant To: CCP4BB@JISCMAIL.AC.UK Date: Tuesday, 23 December, 2014, 2:47 PM

Re: [ccp4bb] obtaining CC1/2 from old log file of hkl 2000

2014-12-23 Thread wtempel
Ansuman, you can calculate arbitrary merging stats, including CC1/2, Rmeas from *unmerged* intensities, with a program like AIMLESS http://www.mrc-lmb.cam.ac.uk/harry/pre/aimless.html#data_from_denzo. By default, HKL2000 (scalepack) will have output *merged* intensities, but merging is inherently