Hi Xtallographers,
I have been able to purify a protein that was initially going into
inclusion bodies from the excellent suggestions that I got here.
So my lysis buffer has 0.5M Guanidium Hydrochoride, 2% TritonX-100, 500mM
NaCl, 5% Glycerol in 20 mM Tris-HCL pH 8.0
The problem is that the
In addition to what others have -- correctly -- stated I want to add one
more thing:
Yes, you are right, if you do not get your hands on a monitor with
built-in emitter, you'll need ad least a K4000 and in many cases the
VESA din bracket (~$50). You do not have to buy the expensive ($800+) 3D
Dear all,
Is there any reason for using Babinet scaling for bulk solvent correction
instead of mask based scaling?
Armando
Hi Ivan
this will not be an answer to your question, but did you consider not
concentrating 'too much' your sample? It happened few times to me that the
protein was precipitating when concentrating for SEC because of the presence of
other impurities. Trying the good old AS precipitation helped
Hi, Harry,
(Happy New Year)
In fact, yes, the ‘what’s new’ sessions are being webcast: I see it fine and
dandy. Hopefully, the problem encountered / reported by Tereza is overcome by
now.
Very Best Regards,
Marcus.
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf
Now it works. Thanks for all advices.
Tereza Skalova
2015-01-08 10:37 GMT+01:00 Tereza Skalova t.skalova.c...@gmail.com:
Do you see the video? I see nothing.
Tereza Skalova
2015-01-07 15:32 GMT+01:00 Ronan Keegan ronan.kee...@stfc.ac.uk:
Dear All,
The webcast for this year's CCP4
Hi,
I watched the live stream of what's new sessions without
any problem from my university's network. The video uses RTMP
streaming, not HTTP. Some network might have difficulty accessing it.
The recordings will be provided in RTMP and HTTP, if they don't change
settings from 2014.
Best
Do you see the video? I see nothing.
Tereza Skalova
2015-01-07 15:32 GMT+01:00 Ronan Keegan ronan.kee...@stfc.ac.uk:
Dear All,
The webcast for this year's CCP4 Study Weekend on Advances in
Experimental Phasing taking place on the 8/9 of January will be available
from the STFC Webinars
I'm not sure the what's new sessions are webcast - try again when the main
sessions are due to start at 11 am local time (in about 25 minutes time).
Harry
--
Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue,
Cambridge Biomedical Campus, Cambridge, CB2 0QH
Chairman of
Yes, this may be the reason. I solved my problem to see the video by
changing house.
It did not work on any computer at work (Windows7, Linux). It works at home
(Windows 8.1 and different network).
Tereza Skalova
2015-01-08 12:39 GMT+01:00 Takanori Nakane
takanori.nak...@bs.s.u-tokyo.ac.jp:
I'll second Jim on this. Just this week, I set up my linux box with the nvidia
3d vision 2 kit which I use with the ASUS VG278HE. My graphics card is the
quadro K4000 and the only pitfall was that I had to purchase the 3 pin
connector to get it to sync:
Yes, this is great info and thoughts. What I still do not understand, however,
is why the noise from air/loop scattering is so bad--why not make sure only the
top of the Gaussian is engulfing the crystal, and the tails can hit air or
loop? Isn't the air scattering noise easily subtractable,
Hi everyone,
Slightly off topic here but I got myself volunteered by my 2nd grade son to
do a show and tell at his class. I have the rock candy experiment ready
with some background info on what I do.
Can anyone direct me to some resources or your personal demo's that you
have done?
Thanks a
Dear all,
I am looking again at 3D monitors. Last year I bought for my old lab the
VG278HR and the PNY K600, as advised by the CCP4BB. (The 3D test images
from Nvidia were running fine under Windows, but I did not get around to
finish the set up with pymol and coot under linux.)
Now at a new
Hi Tobias,
You most definitely do not need a built-in emitter for your system to
work. Instead you use the little emitter temple that comes with a set of
the glasses
http://www.nvidia.com/object/product-geforce-3d-vision2-wireless-glasses-kit-us.html.
In Windows, the emitter can be driven via
This one is probably above second grade, but the equipment setup is
pretty easy
http://ipl.physics.harvard.edu/wp-uploads/2013/03/15c_s07_5.pdf
Measuring the wavelength of light with a ruler
On 01/08/15 13:35, John Lee wrote:
Hi everyone,
Slightly off topic here but I got myself
When my daughter was in Kindergarten, her class took a trip to our
facility, and I showed them some of my crystal trays (What do you see
here? Do you see anything? Clear drops ..., they effectively said).
Then I showed them through the microscope several crystals, and I was
pleasantly surprised
I like the sodium acetate crystallization from Dr. Shakhashiri’s Chemical
Demonstrations. I prepare the supersaturated solution ahead of time, then seed
it with crystals during class. It’s fast, simple, entertaining, and the flask
gets warm, showing that heat is being released by the
I have had young ones grow lysozyme crystals in just a few minutes, using
eye droppers and petri dishes. The crystals grow very fast, you can watch
them grow in the microscope. Also they grow large enough you can see them
by eye. Some izit dye would be fun to add (never did that). Then I let
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