[ccp4bb] Deadline for rapid access proposals at eBIC@Diamond

Dear all – we are extending the 1st January 2017 deadline for rapid access proposals at eBIC to the 8th January 2017 (http://www.diamond.ac.uk/Science/Integrated-facilities/eBIC/How-to-apply.html) Happy christmas Martin -- This e-mail and any attachments may contain confidential, copyright

Re: [ccp4bb] Crystal with ZERO diffraction

I call these crystals as examples of Space Groups 231 where all reflections are systematically absent. Happy Solstice to everyone. The will be more daylight tomorrow (Northern Hemisphere). On Wed, Dec 21, 2016 at 1:44 PM, Tom Huxford wrote: > Dear ccp4b collective mind

[ccp4bb] [Job] Postdoctoral Fellow Position in Structural Bioinformatics at UC San Diego

We are looking for a highly motivated postdoc as part of our new project “Compressive Structural Bioinformatics” funded by the US National Institutes of Health (NIH) Big Data to Knowledge (BD2K) initiative. The Challenge: To enable efficient research on the rapidly growing number of 3D

Re: [ccp4bb] Crystal with ZERO diffraction

I have learned to refrain from catapulting my crystals. Happy holidays, God bless us ... everyone (with diffracting crystals) On Wed, Dec 21, 2016 at 2:35 PM, Keller, Jacob wrote: > >In a second case we were working on a beamline on the west coast of the > US. Every

Re: [ccp4bb] Crystal with ZERO diffraction

Just my 2 cents worth, I am with others in that this is actually not unusual. Outside of what Patrick said the first thing to look at if you can is does the crystal diffract at all. You can do this by taking a room temperature shot, the Mitegen loop/sleeve/pins work well for a quick shot or

Re: [ccp4bb] Crystal with ZERO diffraction

Sadly, I have seen numerous examples of reasonably-sized crystals that give no observable ordered diffraction (I shot a few this weekend, in fact). I can’t give you evidence for what is happening, but I guess that you can build a macroscopic assembly using lattice interactions that are only

Re: [ccp4bb] Crystal with ZERO diffraction

>In a second case we were working on a beamline on the west coast of the US. >Every crystal we put on vanished before we could even collect data - the loops >appeared empty as soon as we looked through the microscope (this sets the >decade). The beamline had an extra strong magnet on the

Re: [ccp4bb] Crystal with ZERO diffraction

Hi Tom et al., While probably unrelated, as a fresh graduate student we did one experiment where we observed no diffraction signal at all - no beamstop shadow, solvent ring etc. Being clever (we thought), we ran burn paper from the slits into the hutch, then through the hutch step by step

[ccp4bb] Crystal with ZERO diffraction

Dear ccp4b collective mind and experience, Greetings from San Diego. I have done my fair share of synchrotron data collection on many diverse macromolecular crystal systems. But this weekend was the first time that I ever shot crystals that failed to diffract entirely. Details: we have

Re: [ccp4bb] Atom clashes in active site?

Hi Andrew, Here are those references: for CH...O hydrogen bonds I'd recommend our review: "Carbon-Oxygen Hydrogen Bonding in Biological Structure and Function" (2012) http://www.jbc.org/content/287/50/41576.full for chalcogen bonds, I don't know of a great recent review, but this recent article

[ccp4bb] Postdoc position available at Emory University, Atlanta GA, USA

A postdoctoral position at Emory University is available immediately on an NIH-funded project investigating bacterial antibiotic-resistance ribosomal RNA methyltransferases. We use interdisciplinary approaches including structural biology, biochemistry, molecular biology and microbiology to

[ccp4bb] SeMet in S2 cell

Dear All, Does anybody have experience with SetMet protein in S2 cells? What kind of reagent should I use and is anything else I should pay attention to? Thanks in advance. Best, Xianchi

[ccp4bb] PhD studentships at Universiy of Leicester

The newly founded (and exceptionally wonderful) Leicester Institute for Structural & Chemical Biology has funded PhD studentships available for September 2017 start Please see http://www2.le.ac.uk/institutes/liscb/study-at-liscb

[ccp4bb] iNEXT course: “X-ray and neutron diffraction studies of macromolecules: from data collection to structures” (Oulu, Finland, May-15 to May-19, 2017)

Dear All, We are pleased to announce our iNEXT course: “X-ray and neutron diffraction studies of macromolecules: from data collection to structures”. This intensive course is organized from May-15 to May-19, 2017 at the Faculty of Biochemistry and Molecular Medicine, University of Oulu in