Re: [ccp4bb] protein quasicrystals?

Hi Yu, Have you tried using the Additive Screen (e.g. from Hampton Research) on top of your crystallization condition, and varying the ratio between protein complex and crystallization solution? From the diffraction pattern, it looks you are almost there. Salt crystals don't show spots close to

Re: [ccp4bb] protein quasicrystals?

Some of the high intensity, high resolution spots are possibly from ice. Diana ** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX

Re: [ccp4bb] protein quasicrystals?

I would say definitely not a quasicrystal, just multiple crystals, but the spots look like salt spots to me, being very sharp and intense. The puzzling thing is that the low-resolution zone is so populated, which would argue a bit against salt. JPK

Re: [ccp4bb] protein quasicrystals?

I also would love to see an image…. JPK + Jacob Pearson Keller Research Scientist / Looger Lab HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 Desk: (571)209-4000 x3159 Cell: (301)592-7004

Re: [ccp4bb] protein quasicrystals?

There are programs which are good at indexing patterns from multiply twinned crystals. Bruker AXS has one, to my knowledge. There may be other sources. I suggest you try that first before you invoke a quasicrystal explanation. James Phillips On Tue, Feb 13, 2018 at 12:07 PM, Takanori Nakane

Re: [ccp4bb] protein quasicrystals?

Hi, "dials.reciprocal_space_viewer" is very useful to identify multiple lattices. For quasicrystal and modulated crystals, "dials.rs_mapper" is also very useful. Best regards, Takanori Nakane > Have you tried microseeding of these sphere crystals? It may help to get > better crystals. > > >

Re: [ccp4bb] protein quasicrystals?

Have you tried microseeding of these sphere crystals? It may help to get better crystals. Burak From: CCP4 bulletin board on behalf of Yu Qiu Sent: 13 February 2018 15:09:43 To: CCP4BB@JISCMAIL.AC.UK Subject:

[ccp4bb] Research Technician Vanderbilt University.

Colleagues, The Spiller lab in the Department of Pharmacology at Vanderbilt University is seeking a Research Assistant to assist Dr. Professor Spiller in his laboratory. The Spiller lab is a structural biology lab focused on infectious disease and the host’s immune response. The job requires a

Re: [ccp4bb] protein quasicrystals?

I am very interested in this topic, and have found incommensurately modulated crystals and few examples of possible protein quasicrystals. Do you have any images you could share? On Tue, Feb 13, 2018 at 9:09 AM, Yu Qiu wrote: > Hi, > > > > I have been trying to crystallize a

[ccp4bb] protein quasicrystals?

Hi, I have been trying to crystallize a protein complex and keep getting sphere shape crystals. The diffraction is around 3 angstrom, but looks like multiple lattices. I am wondering if it could be a quasi crystal? Is there anyone has such experience? Thanks, Yu

[ccp4bb] PhD Positions in Integrative Structural Biology in Vienna

*International PhD Program in **Integrative Structural Biology (ISB)* *www.phd-structural-biology.at invites applications for**PhD studentships ** *** The ISB Program offers a unique training and research network to study the structure and mechanism of complex macro-molecular systems involved