Re: [ccp4bb] tNCS incompatible with cell dimensions

2019-05-31 Thread Boaz Shaanan
Wouldn't c222 or c222(1) be included in the Phaser run if all orthorhombic sg's were requested? Boaz Boaz Shaanan, Ph.D. Department of Life Sciences Ben Gurion University of the Negev Beer Sheva Israel On May 31, 2019 23:37, Diana Tomchick wrote: Your native Patterson

Re: [ccp4bb] tNCS incompatible with cell dimensions

2019-05-31 Thread Diana Tomchick
Your native Patterson indicates pseudo C-centering. Are you sure you don’t have space group C222(1)? If your space group is correct, it’s still pseudo C-centered. You should see that in the intensity-weighted reciprocal lattice. You could try re-indexing on just the most intense spots to give

[ccp4bb] tNCS incompatible with cell dimensions

2019-05-31 Thread Kevin Jude
Hello community, I wonder if I could solicit advice about a problematic dataset. I plan to solve the structure by molecular replacement and expect that the protein is relatively compact, ie not elongated. SAXS data supports this expectation. The crystals diffract to 2.6 Å resolution and appear to

[ccp4bb] Network launch event: Interfaces in Cryo-EM (ICE) on July 3rd

2019-05-31 Thread Jay Bertrand
Hi All, I wanted to signal the event on 'Interfaces in Cryo-EM (ICE)' that will be taking place at the Research Complex at Harwell (RCaH) in Didcot, U.K. on July 3rd. A key goal of the meeting is to build a network for academic researchers interested in Cryo-EM and members of related Industry

Re: [ccp4bb] Unknown density

2019-05-31 Thread Nicholas Keep
There is no blue (2fo-fc) density at least at the current contour level in your difference density.  That probably indicates what you are seeing is noise rather than a real feature. I am also seeing more intense (above 7 sigma in some cases) positive difference density without actual density

[ccp4bb] PhD position at NUI Galway, Ireland

2019-05-31 Thread Crowley, Peter
PhD position at NUI Galway, Ireland The Crowley laboratory is recruiting a PhD candidate to begin in September 2019. This four year fully-funded project is part of SFI research centre SSPC-Pharm5, and includes an annual stipend, registration fees, consumables costs and travel allowance. We