The old saying was degrees, not frames. If your frame width is not 1
degree, the result will differ accordingly.
One factor is whether the detector is centered or offset, and whether it
is large enough to get the entire pattern. If the detector is offset,
you are not getting the full
There are lots of places where you could find this information (many textbooks,
articles, etc.) but one that I use for classes is quite good due to ease of
understanding. It’s part of the Proceedings of the CCP4 Study Weekend on Data
Collection and Processing. There are other quite excellent
Well, its not no. of frames but minimum degree of crystal rotation. A goggle
search gave me this article.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557013/
Dhiraj
From: CCP4 bulletin board on behalf of Murpholino
Peligro
Sent: Monday, June 22, 2020 5:03
Hi.
Quick question...
I have seen *somewhere* that to get a 'full dataset we need to collect n
frames':
at least 180 frames if symmetry is X
at least 90 frames if symmetry is Y
at least 45 frames if symmetry is Z
Can somebody point where is *somewhere*?
...also...
what other factors can change
A further note on assembly search: since April the RCSB PDB offers
structure search for assemblies. See the "Structure Similarity" section in
https://www.rcsb.org/search/advanced. This works only for PDB-deposited
structures but we are working on making it available for 3D coordinates
submitted by
Thanks everyone for recommendations. The AKTA currently lives in a cold
room hence all of our purifications have been optimised at 4C, and i'm
reluctant to transition them to RT. Also, most of our columns are not
jacketed.
On Sun, 21 Jun 2020 at 18:19, Boniecki, Michal
wrote:
> Hi Alan,
*Postgraduate Research Fellow Position*
*Membrane Structural and Functional Biology – Caffrey Lab*
*Trinity College Dublin, Ireland*
*Post Summary*. The Membrane Structural and Functional Biology group seeks
to solve the structure of medically important membrane proteins for use in
The Multiscale Research Institute for Complex Systems (MRICS) at Fudan
University is located at the Zhangjiang Campus of Fudan University and is
supported by the Shanghai High-level Talents Program. MRICS is strongly
committed to the development of novel and effective multi-scale imaging
Dear Matthew,
at your resolution, 1.7A, you are seeing a large difference in
resolution limits. I'd be curious to know how much "anisotropy" you have
in the single number anisoB. Could you share it?
As to building felxible loops or peptide, I suspect you anisoB to be
mild (<40A2 for your
