[ccp4bb] School on Single molecule biophysics in cell lysates, 13-17 May 2024, IBT, Vestec, Czech Republic

2024-01-07 Thread Jan Dohnalek
Dear colleagues, The registration to a specialized course - Basic level school of MOSBRI is open. Find all the relevant details on this page https://www.mosbri.eu/training/basic-level-schools/bls2/ This basic-level school is aimed at biologists, biophysicists, biochemists, structural biologists,

Re: [ccp4bb] How to make GDP.BeF3 solution ?

2024-01-07 Thread Marian Oliva
Dear Firdous, As Matt refers BeF3 mimics the GTP/ATP bound states. For the transition state we have used mixtures of AlCl3 and HKF2 in combination with GDP (or ADP) and MgCl2 within the buffer. Be aware that depending on the concentrations used of each component you get different proportions

Re: [ccp4bb] Can Refmac5 refine temperature factor residue by group?

2024-01-07 Thread chenzhonghao...@163.com
Dear colleagues, Thank you to everyone who responded. Here is a short summary: 1.Refmac using isotropic B-factors with tight restraints 2. Add a TLS in refinement may help. Best wishes, chenzhonghao...@163.com From: Tom Peat Date: 2024-01-07 10:57 To: CCP4BB Subject: Re: [ccp4bb]