[ccp4bb] Ample fails

: [Errno 8] Exec format error *** -- Dr. Florian Sauer Rudolf Virchow Zentrum für Experimentelle Biomedizin Josef-Schneider-Str. 2, Haus D15 97080 Würzburg

Re: [ccp4bb] effective iodide conc. for SAD data

Dear Rajesh, another method to incorporate Iodine into your crystal is by simply placing a drop of KI/I2 solution next to the crystallization drop. Have a look here: Acta Cryst. (2006). D62, 280-289 New methods to prepare iodinated derivatives by vaporizing iodine labelling (VIL) and hydrogen

Re: [ccp4bb] effective iodide conc. for SAD data

sites? There are two distinct methods based on I2/I-. Florian Sauer wrote: Dear Rajesh, another method to incorporate Iodine into your crystal is by simply placing a drop of KI/I2 solution next to the crystallization drop. Have a look here: Acta Cryst. (2006). D62, 280-289 New methods

Re: [ccp4bb] Off topic: Mammalian gene expression in E. coli

Concerning the contamination of recombinantly expressed proteins with bacterial chaperones, one might wish to try a complete removal using ATP and denatured protein as a bait as described here: Protein Expr Purif. 2000 Oct;20(1):45-7. Separation of copurifying GroEL from

Re: [ccp4bb] Bypassing phase separation for nice crystals.

Original-Nachricht Betreff:Re: [ccp4bb] Bypassing phase separation for nice crystals. Datum: Mon, 18 Jul 2011 17:01:27 +0200 Von:Florian Sauer sa...@embl-hamburg.de An: F. Timur Senguen ftseng...@gmail.com CC: CCP4BB@JISCMAIL.AC.UK Dear Timur, one

[ccp4bb] How to distinguish between Na+ and Mg2+?

Dear CCP4BBers, I'm refining the structure of a Ca-binding protein with several EF-hands against 2.2A data. There is clear density for an ion in several EF-hands coordinated by Asp/Glu, Ser, one backbone O and one water (coordination number 5 and 6). Ca2+ can be excluded as there are no

[ccp4bb] latest XDS only using a single thread

/ data. Does anything have to be specified differently in the new version? Best wishes, Florian -- Dr. Florian Sauer Rudolf Virchow Zentrum für Experimentelle Biomedizin Josef-Schneider-Str. 2, Haus D15 97080 Würzburg

[ccp4bb] [SOLVED] Re: [ccp4bb] latest XDS only using a single thread

-10 13:24, Florian Sauer wrote: Hi Kay, thanks for your quick response! a) I am using BUILT=20170615 on Ubuntu 14.04. b) xds_par c) My machine has 2 cores (4 threads possible). The dataset contains 3600 frames. On a different machine (Opensuse) running an older built (20170215), xds_par uses all

[ccp4bb] PhD position at the Rudolf Virchow Center, Würzburg, Germany

Dear all, we have an opening for a PhD position on hemibodies and their antigen complexes at the Kisker lab. If you're interested, have a look at the full description: https://www.uni-wuerzburg.de/en/rvz/news/jobs-and-career/job-offers/ Best regards, Florian -- Dr. Florian Sauer Rudolf