Re: [ccp4bb] Unidentified electron density

2022-07-02 Thread Sayan Saha
Dear all, As suggested by most people, we modeled Zn2+ ions coordinated with three water molecules. Thanks for everyone’s response. With best regards, Sayan Saha On Fri, Jul 1, 2022 at 8:04 PM Jon Cooper wrote: > Hello, I wonder if it is disorder i.e. alternative conformations of the &g

Re: [ccp4bb] Regarding the correct space group identification

2022-07-28 Thread Sayan Saha
Dear Sir, The crystal-to-detector distance was set to 190 mm. Yes, multiple diffractions seem to be present. We have not yet tried Zanuda on the P1 structure. However, the Rw/Rf of P1 structures are little higher (31/34%). With best regards, Sayan Saha. On Thu, Jul 28, 2022 at 5:22 PM Schreuder

Re: [ccp4bb] Regarding the correct space group identification

2022-07-28 Thread Sayan Saha
gree. Please find attached two diffraction images. With best regards, Sayan Saha. On Thu, Jul 28, 2022 at 9:41 PM Sayan Saha wrote: > Dear Sir, > > The detector-to-crystal distance was 190 mm. The Oscillation range was 1.0 > degree. Please find attached two diffraction images. >

Re: [ccp4bb] AW: [ccp4bb] Regarding the correct space group identification

2022-07-28 Thread Sayan Saha
Dear Madam, The data were processed in C2 and P222 independently from the same diffraction. There are two protomers in the ASU. With best regards, Sayan Saha. On Thu, Jul 28, 2022 at 7:25 PM Eleanor Dodson < 176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk> wrote: > I cant see how th

Re: [ccp4bb] Regarding the correct space group identification

2022-07-29 Thread Sayan Saha
Dear Sir, The Rw/Rf for P22121 structure solution is ~29/32%. For C2 structure solution, it is a little higher, 32/35%. With best regards, Sayan Saha. On Fri, Jul 29, 2022 at 3:25 PM Andrew Leslie - MRC LMB < and...@mrc-lmb.cam.ac.uk> wrote: > Dear Sayan, > >

[ccp4bb] Regarding the correct space group identification

2022-07-28 Thread Sayan Saha
with an Rw/Rf of 29/32% only. The protein is bound to a ligand (co-crystallization) for which a clear density can be observed. Any help and suggestion in this regard would be very helpful. With best regards, Sayan Saha