[ccp4bb] cryo-em data conversion

Dear Members, Sorry for if the question is off topic. Can someone suggest the easiest way to convert .tif file (raw data) into .mrcs format and also need to create a .star file to use Relion for the following steps? Thanks for your help. Best regards, Hena

[ccp4bb] crystallization screen for protein-protein complex

Dear Members, I am trying to crystallize a protein-protein complex. Both are soluble proteins and form complex as observed in FPLC either co-purifying or separately purifying and then mixing with equi-molar ratio. Looking for suggested screens to try for crystallization. So far tried with Qiagen

Re: [ccp4bb] database of crystallization condition

Tel +613 9662 7326 Email janet.new...@csiro.au -- *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Hena Dutta [hdutt...@gmail.com] *Sent:* 25 July 2013 00:36 *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* [ccp4bb] database of crystallization

[ccp4bb] database of crystallization condition

Hi, Can anyone tell, if there is any database containing the crystallization conditions of published structures? I want to see the conditions people have used for those proteins having some structural similarity. Any suggestion would be appreciated. Regards... Hena

[ccp4bb] stereo set up

Hi, My apology for the off topic question. I purchased a Toshiba Qosmio laptop X875Q7390. This has inbuilt ir emiter. It came with NVIDIA 3D VISION active shutter glasses. This is a 3D vision ready laptop. I can see high quality the 3d stereo using this glass for some given still pictures. I want

[ccp4bb] cloning HD

Hello, Sorry for the question if non-ccp4. I am using a HP workstation Xeon Z400. This has both windows and linux Mint. I am using crystallographic programs with stereo setup in coot, o, pymol, vmd…. I bought a new exactly same desktop with all same hardware configurations except the old one has

[ccp4bb] stereo

Hello, Is anyone using nvidia quadro 4000 graphics card for linux environment to see stereo in pymol, coot, O or similar programs? If yes, I like to buy that. Thanks... Hena

[ccp4bb] O in windows

Hi, Is there anyone using O in windows environment with stereo setup? Could you please tell how to setup? I have quadro fx 3700 and able to set up stereo in coot, pymol, vmd in windows env. Thanks... Hena

[ccp4bb] stereo

Dear Members, Is any one using NVidia NVision 3D Setup with active stereo in linux distribution for crystallographic work? Which one would be the best choice to set up, an active stereo or a passive stereo for crystallographic work? Can anyone shade some details on this? I am planning to buy or

[ccp4bb] Protein expression, purification and crystallization

Dear Members of CCP4BB and PHENIXBB, Can you suggest a reliable website where I can get basic and advanced information on protein expression, purification and crystallization. I like to read on the monitor. Thanking you in advance... Hena

[ccp4bb] PYMOL installation in openSUSE 11.3 (64 bit)

Hello, I was trying to install PYMOL in my openSUSE 11.3 as follows: cd /usr/local hena@a:/usr/local sudo svn co https://pymol.svn.sourceforge.net/svnroot/pymol/trunk/pymolpymol cd pymol hena@a:/usr/local/pymol sudo python setup.py install And I got the following error at the end: package

Re: [ccp4bb] PYMOL installation in openSUSE 11.3 (64 bit)

Sorry for my earlier non CCP4 posting in CCP4BB. hena On Mon, Mar 7, 2011 at 9:30 AM, Ben Eisenbraun b...@hkl.hms.harvard.eduwrote: Hello Hena, I was trying to install PYMOL in my openSUSE 11.3 as follows: ... In file included from modules/cealign/src/ccealignmodule.H:57:0,

[ccp4bb] stuck with COOT installation in openSUSE 11.3

Hello, I could not open the COOT GUI after installing either from 'coot-0.6.1-binary-Linux-x86_64-centos-5-gtk2.tar.gz' or from 'coot-0.6.2-pre-1-revision-3205-binary-Linux-x86_64-centos-5-gtk2.tar.gz' I used the following commands: 1. from /usr/loca/src sudo tar xvzf *.gz as suggested in