Re: [ccp4bb] Analysis of NMR ensembles

Hi Harry, The superpose/overlay of all the structures in PyMol should inform you the rigid part of the protein as well as the flexible part. The rigid part would have very low backbone RMSD or overlay tightly and the flexible part (loops, N-term and C-term etc.) would not superpose tightly. If

Re: [ccp4bb] protein oligomer

Keep the protein concentration low during purification steps along with using other anti-aggregation agent/s. Make sure that the pH at which you are purifying is not close to the pI of the protein. Until completely purified, all purification steps should be performed in a cold room if it is a

Re: [ccp4bb] Difficult purification with imac columns

Hi Narayanan,We had similar problems with a membrane protein. It did bind Ni-NTA resin strongly. We washed out the resin (Ni-NTA) with high imidazole to sealer all other proteins and eluted out protein with very small concentration of SDS at room temp with shaking. Please read our paper in- 

Re: [ccp4bb] Problems with an exonuclease

Yes, washing IB with 10 % BPER  twice with sonication makes our IB (from various proteins) very clean before denaturation with 6M guanidine hydrochloride and further processing. Smita  On Wednesday, June 7, 2017 12:03 PM, Nicole Thomas wrote: I've found that

Re: [ccp4bb] Protein precipitation

Hi Manjula, I will lyse the bacterials cells and monitor the degradation of the lysed protein in lysis buffer over 4-5 days before proceeding with purification.   What is the lifetime of this expressed protein once lysed?  How long can it stay after lysis without degradation or aggregation?   

[ccp4bb] Postdoc position in Structural Characterization of a membrane-associated enzyme

Postdoc position in Structural Characterization of a membrane-associated enzyme Lookingfor an outstanding and highly motivatedindividual to start immediately in a postdoctoral position to study structureand mechanism of a membrane-associated enzyme involved in co-translationalmodification. The