Re: [ccp4bb] experiences with cross-polarisers / black-white imaging vs color

2020-10-01 Thread V Nagarajan
Hi Tommi, Obviously for LCP glass plates, color versus black and white should make little difference. For everything else, all darker colors would look more or less the same and lighter colors similarly so not very useful, in my experience. Nagarajan V. Nagarajan JANSi On 10/1/2020 12:19

Re: [ccp4bb] polarizer

2020-08-16 Thread V Nagarajan
Hi, place a linear polarizer below the tray and one above it, rotating the top one until you get the desired view. V. Nagarajan JANSi On 8/16/2020 8:26 AM, Diana Tomchick wrote: It's my understanding that you have two polarizers on your polarizer-microscope--one in the base, and the one

Re: [ccp4bb] Protein or DNA crystals

2017-06-19 Thread V Nagarajan
Hi Joseph, Could you post the UV fluorescence image? Oftentimes, UV absorbing objects that do not fluoresce (such as nucleic acid crystals) show up dark against a brighter background in UV fluorescence. V. Nagarajan JANSi On 6/19/2017 7:20 AM, Joseph Ho wrote: Dear all: I would like

Re: [ccp4bb] FW: [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

2016-11-10 Thread V Nagarajan
I hear so much about the NIH, and it’s terrible.” V. Nagarajan JANSi On 11/9/2016 7:17 PM, William G. Scott wrote: Dear Edward et al: I agree we shouldn’t engage in partisan arguments on the CCP4bb. However, I think it is a mistake, and perhaps a missed opportunity, to ignore politics comp

Re: [ccp4bb] UV-transmissible plate seals 96-well format

2009-02-02 Thread V. Nagarajan
ClearSeal film from Hampton works with our UVEX UV microscope, despite some attenuation of UV light. For hanging drops, Greiner BioOne's films are the best. V. Nagarajan JAN Scientific, Inc. http://janscientific.com -Original Message- From: CCP4 bulletin board [mailto:ccp

Re: [ccp4bb] X-ray photon correlation length

2009-01-29 Thread V. Nagarajan
From memory, correlation length is the length during which the phase of the electric field is preserved. It's typically computed by applying time (pulse width)- frequency (converted to length) uncertainty principle. V. Nagarajan JAN Scientific, Inc. -Original Message- From: CCP4 bulletin

Re: [ccp4bb] offtopic__which crystals to harvest

2009-01-14 Thread V. Nagarajan
Actually, UV fluorescence imaging appears to be a pretty reliable means of discriminating protein crystals, provided your protein has Trp residue(s). V. Nagarajan JAN Scientific, Inc. http://www.janscientific.com -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk

Re: [ccp4bb] UV light source for protein xtal detection

2008-05-15 Thread V. Nagarajan
We are about to release an all-LED based UV microscope for automated scanning of 96-well plates. Please contact me if you are interested. Thanks, V. Nagarajan JAN Scientific, Inc. Seattle, WA, USA. -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Li