Dear all
We may use a Honeybee 963 robot to screen crystallization conditions for
trypsin-containing protein samples and we are worried about robot
contamination by residual protease.
How do you normally clean robots when using this kind of sample? Your
suggestions/recommendations will be
would also
alleviate the protease problem.
Regina
From: Horacio Botti hbo...@pasteur.edu.uy
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, January 24, 2012 7:12 AM
Subject: [ccp4bb] Crystallization robot and trypsin
Dear all
We may use a Honeybee 963 robot
Hi Horacio,
we have a Cartesian Honeybee and perform trypsin-containing trials by setting
up drops with a dedicated protein+trypsin tip, which we then wash extensively
with water, 6 M guanidinium hydrochloride, then water, isopropanol and again
water.
hth,
ciao,
Sebastiano
On Jan 24, 2012, at
On 12-01-24 08:39 AM, Regina Kettering wrote:
We have a Honeybee system but do not usually use
proteases. The biggest problem we have found is that if
anything precipitates in the tips they have to be washed
very well, usually
