Dear CCP4bbers,
I have a protein molecule(~9.0 kDa) that crystallized in the presence of
0.15M KBr and HEPES pH 7.0. Since there is no homologuous structure present,
I intend to perform heavy metal derivatization. I read some literature which
suggested that I could carry out quick soak with 0.5M
Hi Amit,
http://www.doe-mbi.ucla.edu/~sawaya/tutorials/Phasing/references.html
And the (IMHO) seminal heavy atom derivative reference:
Petsko, G.A., Perparation of Isomorphous Heavy-Atom Derivatives
Methods in Enzymology, Volume 114, , pages 147-157.
should give you all the info you need.
The
2008/9/25 amit sharma [EMAIL PROTECTED]
Dear CCP4bbers,
I have a protein molecule(~9.0 kDa) that crystallized in the presence of
0.15M KBr and HEPES pH 7.0. Since there is no homologuous structure present,
I intend to perform heavy metal derivatization. I read some literature which
]
***
- Original Message -
From: amit sharma
To: CCP4BB@JISCMAIL.AC.UK
Sent: Thursday, September 25, 2008 6:03 AM
Subject: [ccp4bb] Quick-soak
Dear CCP4bbers,
I have a protein molecule(~9.0 kDa) that crystallized in the presence of
0.15M KBr and HEPES pH 7.0
Hi Amit,
Dr. Dauter has several excellent papers about solving crystal structures with
halide ions. Use this search string in PubMed (without the quotes) to retrieve them: dauter [AUTH] AND halide. The anomalous signal of iodide can be detected using a home x-ray source but the anomalous
Or you could try crystallizing the protein in the presence of KI or
NaI, and collect some in-house SAD data. You could also try to boost
the concentration and number of ordered halide sites by quick soaking
the crystals with a higher concentration of the iodide salt.
In my limited
We have had really outstanding success using trimethyllead and
samarium acetate as 'quick soak' derivatives. Both are soluble to 100 mM
in most crystallization solutions and have large anomalous signals at the
copper-kalpha wavelength used at most home sources. A quick 30 sec to 5
minute
Hi,
I'd like to find out how successful the quick soak method for heavy
atom derivatisation proposed by Radaev and Sun:
Sun PD, Radaev S, Kattah M. Generating isomorphous heavy-atom
derivatives by a quick-soak method. Part I: test cases. Acta Cryst.
2002. D58:1092-1098.
has been in
Hi,
I do not use their method as such - however, I love heavy atom soaks and
do them any time I can, so I've got very similar experiences in the past.
Heavy atoms can bind very quickly even from quite dilute solutions - the
quickest I've ever soaked (and got useful data) was sodium
Hi - sorry - rather than iodine I meant to say we had had success with
Potassium Iodide (1M for 20 seconds)!
Cheers
James
[EMAIL PROTECTED] wrote:
Hi,
I do not use their method as such - however, I love heavy atom soaks and
do them any time I can, so I've got very similar experiences in
I also solved a structure on our home CuKa source by SIRAS with 20-60sec in
0.5-1M KI.
JPK
==Original message text===
On Tue, 25 Sep 2007 6:55:28 pm CDT James Whisstock wrote:
Hi - sorry - rather than iodine I meant to say we had had success with
Potassium Iodide (1M
11 matches
Mail list logo
