Yes, our new Akta prime has also only 2 mm path length
and readings are, therefore, much smaller than with
1 cm path length in a typical spectro-photometer.
Best
Marius
Hi all,
We recently got low UV reading on our AKTA prime. We contacted GE
healthcare, but was told that the UV reading on
sure the A280 dial is set
correctly on the lamp. Please check the manual for guidance.
Best,
Scott
- Original Message -
From: Matt Colins matt.colins2...@yahoo.commailto:matt.colins2...@yahoo.com
Date: Wednesday, July 1, 2009 4:40 pm
Subject: [ccp4bb] low UV reading on AKTA prime
Hi Matt,
AKTA system does allow you check the how-good percentage of the UV lamp,
so if the UV lamp is new, and it gives low percentage (I do not mean the
20%) then your AKTA system may have the problem like Scott said or other
thing in the Optical path.
There are other possibilities, however!
Hi all,
another reason to get low readings from the UV meter can be a drift
in the baseline. The photometer is properly calibrated only when
you turn it on, I think. Since we have our machine running in the
coldroom without (usually) turning it off for month, we have observed
wrong 260/280
Hi all,
We recently got low UV reading on our AKTA prime. We contacted GE healthcare,
but was told that the UV reading on AKTA prime should be 20% of the
spectrometer reading, because the path length of the flow cell of AKTA prime is
only 2 mm (20% of the pathlength of a spectrometer cuvette).
for guidance.
Best,
Scott
- Original Message -
From: Matt Colins matt.colins2...@yahoo.com
Date: Wednesday, July 1, 2009 4:40 pm
Subject: [ccp4bb] low UV reading on AKTA prime
To: CCP4BB@JISCMAIL.AC.UK
Hi all,
We recently got low UV reading on our AKTA prime. We contacted
GE
the A280 dial is set correctly on the lamp. Please
check the manual for guidance.
Best,
Scott
- Original Message -
From: Matt Colins matt.colins2...@yahoo.com
Date: Wednesday, July 1, 2009 4:40 pm
Subject: [ccp4bb] low UV reading on AKTA prime
To: CCP4BB@JISCMAIL.AC.UK
Hi all,
We
