Re: [ccp4bb] Off topic: Flourescence anisotropy measurement

2017-07-23 Thread Smith Liu
May i ask, whether the fluoresnce anisotropy method was reliable enough to determine the stoichiometry of a protein complex? 发自网易邮箱大师 在2017年07月22日 03:44，Phoebe A. Rice 写道： You might also be getting aggregation. If you do an old-fashioned EMSA ("gel shift") assay, does it hang up in the well?

[ccp4bb] Wellcome Trust postdoctoral fellowships available at the University of Oxford

2017-07-23 Thread Tom Burnley
Dear all, Posted on behalf of Peijun Zhang... THREE Wellcome Trust funded postdoctoral fellowships are available in Peijun Zhang lab at the University of Oxford (https://www.ndm.ox.ac.uk/prin cipal-investigators/researcher/peijun-zhang

Re: [ccp4bb] Off topic: Flourescence anisotropy measurement

2017-07-23 Thread Mohammad Khan
Dear Thomas, I do all my experiments in a 384 well, non-binding plates. I have a salt concentration of 50 mM NaCl, with BSA. i will try the other suggestions as well. >From a couple of experiments, I could determine the Kd to be approx. 10 nM. Thanks for all the suggestions! On Fri, Jul 21,

Re: [ccp4bb] Off topic: Flourescence anisotropy measurement

2017-07-23 Thread Mohammad Khan
Dear Didier and Opher, The difference in polarities can reach upto 60 units. I have tried concentrations between 1-5 nM DNA. Maybe I will give higher concentrations a shot. Thanks! On Fri, Jul 21, 2017 at 4:02 PM, Didier Spittler wrote: > Hello, > > What is your

Re: [ccp4bb] Off topic: Flourescence anisotropy measurement

2017-07-23 Thread Mohammad Khan
Dear Julius, That is a good suggestion. I will definitely try this. Thanks! On Fri, Jul 21, 2017 at 3:41 PM, Rabl, Julius wrote: > Dear Mohammad, > your buffer is key to success in biophysical measurements. While your > protein may be totally fine in standard buffer, the