Dear all,
I just ran into this problem and would like to see if I could get some
helpful tips before my protein completely crashes out.
I have a protein as 6His fusion and it remained bound to the Ni resin with
40 mM Imidazole wash (added to 1XPBS) but then was eluted off with 200 mM
(added to
Thank you all for the quick replies - I'm trying many of the tips in small
batches and would send out a feedback as soon as appropriate, gratefully, -
J.J.
On 2/14/08, Jacob Wong [EMAIL PROTECTED] wrote:
Dear all,
I just ran into this problem and would like to see if I could get some
helpful
), but diluting with 1XPBS (or H2O) doesn't help clear the precipitate -
so it seems that the key things here are imidazole, trace amount of nickel,
and a good buffer.
Thank you so much for all your help, caring and patience. This is a great
community.
Have a nice weekend, -J.J.
On 2/14/08, Jacob
: Refmac complains not to have such a spacegroup.
How do I get around with this, without a trip back to the HKL2000? Thank
you!
Jacob Wong
Dear all,
http://www.nature.com/nature/journal/v458/n7234/fig_tab/nature07819_F3.html
Just came across this figure Fig. 3b) and would like to know what is the
general and easiest way to make things like this, as a non-brainer. Thank
you, -Jacob
a pointer. Happy Chinese New Year!
Thanks, Jacob Wong
/nature/journal/v425/n6959/full/nj6959-746a.html
Thank you, Jacob Wong
On Sat, Jan 29, 2011 at 12:47 AM, Jacob Wong jacob.j.w...@gmail.com wrote:
Dear All, sorry for this off-topic inquiry. I am interested in knowing
about the current scientific research in Singapore but have had hard time
finding
real-time, crystallography and beyond. With my best wishes for a
wonderful and prosperous Chinese New Year - to researchers all over the
world. Sincerely yours, Jacob Wong
On Sat, Jan 29, 2011 at 7:11 AM, Jacob Wong jacob.j.w...@gmail.com wrote:
Thanks to those who has responded to me and asked
along, prohibiting me to do real-space refinement effectively. How to get
around with this? Thank you!
Also, are there any easily accessible programs that could help me build
these side-chains based on the very good experimental density map currently
at 2.3A?
Thanks, Jacob Wong
Change Chain ID into the Renumber Residues window so
that I I could do both changes on the fly? Thanks, Jacob Wong
On Wed, May 4, 2011 at 3:59 AM, Tim Gruene t...@shelx.uni-ac.gwdg.de wrote:
Dear Jacob Wong,
have you tried arp warp for model building? It should do a reasonably good
job
at 2.3A
,
carboxypeptidase A/B/Y, aminopeptidase, Glu-C, and others that you may
recommend. I wish to purchase like 1 mg for each of these so that I could
make 1 mg/ml stocks and 10X series dilutions. Thanks in advance for your
advice. Sincerely yours, - Jacob Wong
Dear all, sorry for this off-topic subject. I had this link below functional
until late last month, with the April 31st issue of Science and issues
thereafter not being fed any longer. I believe someone out there may have a
quick solution for me? I tried to contact them to not avail. Thanks in
Dear colleagues, please help me with a CNS syntax. In the refine.inp, I
could provide as many topology (or parameter) files as necessary:
{refine.inp}
{* topology files *}
{===} topology_infile_1=CNS_TOPPAR:protein.top;
{===} topology_infile_2=CNS_TOPPAR:dna-rna.top;
{===}
encountered: program will be aborted.
(CNS is in mode: SET ABORT=NORMal END)
On 7/21/09, Jacob Wong jacob.j.w...@gmail.com wrote:
Dear colleagues, please help me with a CNS syntax. In the refine.inp, I
could provide as many topology (or parameter) files as necessary:
{refine.inp
Dear all, I came across an online program long way back that I could not
longer remember (embarrassing...). But there should be a program that will
take in my protein sequence and generate a list of charge residues that may
be solvent exposed for potential mutation to improve solubility or
Got answers in a matter of minutes, thanks to all!!! -jacob
http://nihserver.mbi.ucla.edu/SER/
On 2/12/10, Jacob Wong jacob.j.w...@gmail.com wrote:
Dear all, I came across an online program long way back that I could not
longer remember (embarrassing...). But there should be a program
Dear all,
Just would love to know what else is out there in addition to ELBOW in
Phenix?
My utility is to generate the .pdb and .cif files from a SMILES string that
would allow me to do compound fitting and real-space refinement in coot,
and then reciprocal refinement elsewhere (assuming I won't
Thanks Paul - I love the ccp4 suite. I'm interested in knowing if there is
any other solution out there that may be more economical in licensing,
especially if I only go as far as coot as a "commercial user".
Are you suggesting that coot may not be a freeware anymore in the near
future?
Yours,
Thanks Paul for addressing my inquiry late hours. Kudos to your huge
contributions to the vast crystallography community. All my best wishes,
Jacob
On Sun, Mar 22, 2020 at 11:15 PM Paul Emsley
wrote:
> On 23/03/2020 03:05, Jacob Wong wrote:
> > I love the ccp4 suite. I'm interested i
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