Re: [COOT] problem with refining DNA

2010-02-04 Thread Miguel Ortiz Lombardia
Le 4 févr. 2010 à 04:37, Norman Zhu a écrit : hello there I am in the process of refining a protein structure complexed to DNA promoter site. I ran into difficulty as i try to move a few bases into patch of electron density that is obviously meant for them. dragging the bases with

Re: [COOT] problem with refining DNA

2010-02-04 Thread Paul Emsley
Miguel Ortiz Lombardia wrote: Le 4 févr. 2010 à 04:37, Norman Zhu a écrit : hello there I am in the process of refining a protein structure complexed to DNA promoter site. I ran into difficulty as i try to move a few bases into patch of electron density that is obviously meant for

Re: [COOT] problem with refining DNA

2010-02-04 Thread Mark A. White
FYI, I just used a Matrix Weight of 2.0e-5, yes that's a weight of 0.2, on a rather poor 2.9A map generated from a PHS file (XtalView format text filecreated from the CNS omitmap.coeff map output). I have not noted any real problems with REFMAC generated MTZ files, so the weighting must be