>> TPP v4.0 JETSTREAM rev 2

There's a newer release, perhaps the problem has already been resolved.

-----Original Message-----
From: spctools-discuss@googlegroups.com
[mailto:[EMAIL PROTECTED] On Behalf Of marpello
Sent: Tuesday, November 18, 2008 3:33 AM
To: spctools-discuss
Subject: [spctools-discuss] Problem with ProdID and TPP


Hi all,
I installed ProdID and TPP on two different computers.
I have download my database and I have run ProbID. Then I converted
the file.out to file.xml.
In this file I changed the database path and I pointed it on the same
database installed on TPP computer. I run xinteract without
PeptideProphet and the commad successful, but the file generated is
empty. Moreover if I run PeptideProphet the command failed with the
follow messagge:
----------------------------------
xinteract (TPP v4.0 JETSTREAM rev 2, Build 200807011544 (MinGW))

running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/
Inetpub/wwwroot/ISB/data/example.xml""
 file 1: c:/Inetpub/wwwroot/ISB/data/example.xml
 processed altogether 0 results


 results written to file c:/Inetpub/wwwroot/ISB/data/interact.pep.xml

 direct your browser to http://localhost/ISB/data/interact.pep.shtml



command completed in 1 sec

running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05 EXTRAITRS=20"
 (PROBID)
MS Instrument info: Manufacturer: UNKNOWN, Model: UNKNOWN, Ionization:
UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN


command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05 EXTRAITRS=20" failed: Unknown error

command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05 EXTRAITRS=20" exited with non-zero exit code: -1073741819
QUIT - the job is incomplete

Command FAILED
--------------------------------------------------

So, I'd like to know where is the error?!
What kind of file I must submit to xinteract?!
I paste my file.xml
-------------------------------------------------------
<?xml version="1.0" encoding="UTF-8"?>
<?xml-stylesheet type="text/xsl" href="/data3/search/akeller/
pepXML_protXML/trans_proteomic_pipeline/pepXML_std.xsl"?>
<msms_pipeline_analysis date="2008-11-18T12:51:27" xmlns="http://regis-
web.systemsbiology.net/pepXML" xmlns:xsi="http://www.w3.org/2001/
XMLSchema-instance" xsi:schemaLocation="http://regis-
web.systemsbiology.net/pepXML /data3/search/akeller/pepXML_protXML/
trans_proteomic_pipeline/pepXML_v18.xsd"  summary_xml="example.xml">
 <msms_run_summary base_name="/home/marco/Desktop/probIDexample"
raw_data_type="raw" raw_data=".mzXML">
  <sample_enzyme name="Trypsin">
   <specificity sense="C" cut="KR" no_cut="P"/>
  </sample_enzyme>
  <search_summary base_name="/home/marco/Desktop/probIDexample"
search_engine="PROBID" precursor_mass_type="average"
fragment_mass_type="average" out_data_type=".out" out_data=".tgz"
search_id="1">
   <search_database local_path="C:\Inetpub\wwwroot\ISB\data\dbase
\fusarium_oxysporum_f._sp._lycopersici_2_proteins.fasta" type="AA"/>
   <enzymatic_search_constraint enzyme="Trypsin"
max_num_internal_cleavages="0" min_number_termini="2"/>
   <parameter name="precursor_mass_tolerance" value="3.0"/>
   <parameter name="fragment_mass_tolerance" value="1.0"/>
   <parameter name="ion_series" value="0 1 0 0 0 0 0 1 0"/>
   <parameter name="num_output_lines" value="10"/>
  </search_summary>
 </msms_run_summary>
</msms_pipeline_analysis>
------------------------------------------

thanks,
marco


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