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Assuming you are keeping the organization name the
same, no you do not need to run it again. Domain prep needs to be run once for
each domain that exists. It's a very quick thing that creates the groups for
you. If you will not have an Exchange server in a domain, it still doesn't hurt
to run it. This will give you the group structure that you may want to use
anyway.
Marc Zukerman
Senior Network Engineer
Greenwich Technology Partners
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