Have you tried disabling windows authentication to see if the issue
remains?
It could be the underlying conenction that is timing out which is like
the session expiring.
once the connection is expired it is no longer authenticated, it is
dead.

I know I am using integrated authentication and have had the page up
for 2 hours plus with no time out

it could be the connectionTimeout property.

Here are instructions on how to modify this setting...
1) In IIS Manager, expand the local computer, expand the Web Sites or
FTP
Sites folder, right-click a Web or FTP site, and click Properties.
2) On the Web Site or FTP Site tab, in the Connection timeout box, type
the
maximum number of seconds that IIS should maintain an idle connection
before
resetting the connection.
3) For the WWW service, verify that the Enable HTTP Keep-Alives box is
selected. For more information, see Enabling HTTP Keep-Alives.
4) Click Apply, and then click OK.



also see this post (where I got the info from)
http://groups.google.ca/group/microsoft.public.dotnet.framework.aspnet.webservices/browse_thread/thread/2d3ba446d04d760c/14a1a7a467c86441?lnk=st&q=asp.net+401+error+timing+out&rnum=1&hl=en#14a1a7a467c86441


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