What is the name of your custom CDF, and what does print(cdf) report
after loading it with cdf <- AffymetrixCdfFile$byName(...)?

/Henrik

On Wed, May 4, 2011 at 3:56 PM, Fong <fongchunc...@gmail.com> wrote:
> Hi all,
>
> I've created a customCDF following the procedure listed at:
> http://www.aroma-project.org/node/40
>
> The customCDF I made was based on the Brainarray group CDF (ense14 for
> Human Exon arrays to be exact).  I wanted to create a CDF which I
> could run FIRMA on since the customCDF provided by the BrainArray
> doesn't appear map the probeset (group ids) onto the appropriate gene
> (unit IDs) which is necessary to run FIRMA.  I create one that works
> in the flat2CDF() script provided by aroma.affymetrix and then when I
> used it on aroma affymetrix I got the following error:
>
> 20110504 15:46:17|     Setting up ExonChipEffectFile...done
> 20110504 15:46:17|    Retrieving chip-effect #19 of 20 (8_4N)...done
> 20110504 15:46:17|    Retrieving chip-effect #20 of 20 (9_5T)...
> 20110504 15:46:17|     Setting up ExonChipEffectFile...
> 20110504 15:46:17|      Pathname: plmData/
> gardina_colon_cancer,ense14,QN,RMA,merged/HuEx-1_0-st-
> v2/9_5T,chipEffects.CEL
> 20110504 15:46:17|     Setting up ExonChipEffectFile...done
> 20110504 15:46:17|    Retrieving chip-effect #20 of 20 (9_5T)...done
> 20110504 15:46:17|   Retrieving chip-effects from data set...done
> 20110504 15:46:17|  Getting chip-effect set from data set...done
> 20110504 15:46:17|  Updating ExonChipEffectSet...
> 20110504 15:46:17|   Scanning for and applying sample annotation
> files...
> 20110504 15:46:17|    Defining 0 files...
>
> 20110504 15:46:17|    Defining 0 files...done
> 20110504 15:46:17|    No sample annotation files found.
> 20110504 15:46:17|   Scanning for and applying sample annotation
> files...done
> 20110504 15:46:17|  Updating ExonChipEffectSet...done
> 20110504 15:46:17|  Identifying non-fitted units in chip-effect
> file...
> 20110504 15:46:17|   Pathname: plmData/
> gardina_colon_cancer,ense14,QN,RMA,merged/HuEx-1_0-st-
> v2/9_5T,chipEffects.CEL
> 20110504 15:46:17|   Found indices cached on file
> 20110504 15:46:17|   Reading data for these 442179 cells...
> Error in readCel(getPathname(this), indices = idxs, readIntensities =
> FALSE,  :
>  Argument 'indices' is out of range [1,308580].
> Calls: fit ... findUnitsTodo -> findUnitsTodo.ChipEffectFile ->
> readCel
> 20110504 15:46:17|   Reading data for these 442179 cells...done
> 20110504 15:46:17|  Identifying non-fitted units in chip-effect
> file...done
> 20110504 15:46:17| Identifying non-estimated units...done
> 20110504 15:46:17|Fitting model of class ExonRmaPlm...done
>
> Has anyone run into this error before?  Any ideas on what I am doing
> wrong?
>
> Thanks,
>
> Fong
>
> --
> When reporting problems on aroma.affymetrix, make sure 1) to run the latest 
> version of the package, 2) to report the output of sessionInfo() and 
> traceback(), and 3) to post a complete code example.
>
>
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-- 
When reporting problems on aroma.affymetrix, make sure 1) to run the latest 
version of the package, 2) to report the output of sessionInfo() and 
traceback(), and 3) to post a complete code example.


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