I've been attempting to curate an annotation from a Maker produced .gff3 using
Artemis. I'm having a couple of issues:
1) Whenever a value other than "0" or "." is entered in column 8 (holds
"phase" data for CDS features), the feature does not display in the proper
reading frame. This only appears to affect the visuals..the feature
information appears correct. Editing the visual to produce the correct picture
in the correct frame causes the coding region to shift incorrectly. Basically,
if the visual is correct, the feature info is wrong, and visa versa.
2) When I save/edit the gff3 (i.e. create or edit features) in Artemis, the
file does not re-open properly--the gene models are lost. In addition, once
edited, the gff3 cannot be opened in Apollo either.
Any help or guidance would be greatly appreciated.
Shawn Starkenburg, Ph.D.
Los Alamos National Laboratory
PO Box 1663, MS M888
Los Alamos, NM 87545
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