Johan,

brilliant, I had a strong feeling there would be a way to do it (I have faith
in BASE).  will check that out tomorrow.

thanks for putting the merging plugins up too.

much appreciated/tusen tack!

Johan Enell writes:
 > Missed to answer you other questions...
 > 
 > You use the gene filter "In # of Assays"
 > 
 > Exampel:
 > You have 4 assays and you want to filter out the spots with ratio 
 > greater then 2 in at least 3 assays.
 > 
 > You need two criterias for this. The spot filter "ratio > 2" and the and 
 > the gene filter "In # Assays > 2"
 > 
 > /Johan
 > 
 > 
 > Bob MacCallum wrote:
 > > Hi all,
 > >
 > > In the analysis steps part of BASE 1.2.x, is there a way to create a 
 > > reporter
 > > list such that X out of Y assays satisfy some filter condition,
 > > where 1 < X < Y ?
 > >
 > > I already know how to do it where X=1 or X=Y.  For example:
 > >
 > > 1. Reporters where at least one assay has a twofold change
 > > Filter on "ratio not between 0.5, 2", create reporter list R with results,
 > > then filter original set on "reporter list present in R".
 > >
 > > 2. Reporters where *all* assays have max intensity >= 100:
 > > Filter on "max(Ch1,Ch2) < 100", create reporter list S with results,
 > > then filter original set on "reporter list absent in S".
 > >
 > > Quite often you want to filter somewhere in between, i.e. at least half of 
 > > the
 > > assays should show X-fold changes, or whatever.
 > >
 > > It would not be hard to write a plugin to do this, but I haven't written 
 > > any
 > > plugins yet.  So I wondered if there were any GUI tricks I had missed.
 > > Failing that I'll write a pseudo-plugin (a script which takes a custom BASE
 > > file and dumps a reporter list) and worry about configuring it as a plugin 
 > > at
 > > a later date.
 > >
 > > Also, if anyone has an 'averaging over replicates' plugin (based on a
 > > specified sample annotation), I would be very grateful.  Installing such a
 > > plugin would help me figure out how to write one.
 > >
 > > cheers,
 > > Bob.
 > >
 > >   
 > 
 > 
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-- 
Bob MacCallum | VectorBase Developer | Kafatos/Christophides Groups |
Division of Cell and Molecular Biology | Imperial College London |
Phone +442075941945 | Email [EMAIL PROTECTED]

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