Greetings!

The complete stack trace for the error is below[1]. I'm working on putting
sample images from genepix 6.0 somewhere visible - I'm concerned about
confidentiality and so forth so I'm talking with the scientists.
<time passes>
OK, I got the ok from Molecular Devices to post some of their sample data
but it seems that they changed the format since the sample images were
developed because the multiimage tiff here:
$ identify Yeast1.tif
Yeast1.tif[0] TIFF 1928x1749 1928x1749+0+0 PseudoClass 65536c 12.9mb 0.420u
0:02
Yeast1.tif[1] TIFF 1928x1749 1928x1749+0+0 PseudoClass 65536c 12.9mb
does not match the multi-image tiff below.

I'm going to analyze this array and try to put it into base as single-image
tiffs but I need to add  the Yeast reporters from the galfile. Whew!

Anyway...


For my *real* data, this is what identify tells me about the images:
$ identify 13298608_*
13298608_532-uncompressed.tif TIFF 1884x5472 1884x5472+0+0 DirectClass
29.5mb 0.420u 0:02
13298608_635-uncompressed.tif[1] TIFF 1884x5472 1884x5472+0+0 DirectClass
29.5mb 0.440u 0:02

and for the genepix multi-image tiff:
$ identify 13298608.tif
13298608.tif[0] TIFF 495x1396 495x1396+0+0 PseudoClass 65536c 35.5mb 2.510u
0:11
13298608.tif[1] TIFF 495x1396 495x1396+0+0 PseudoClass 65536c 35.5mb 2.440u
0:11
13298608.tif[2] TIFF 1884x5472 1884x5472+0+0 PseudoClass 65536c 35.5mb
2.380u 0:11
13298608.tif[3] TIFF 1884x5472 1884x5472+0+0 PseudoClass 65536c 35.5mb
1.450u 0:09

Note that this spotfinding process (with single image tiffs) doesn't ALWAYS
fail. I built this table to show success:
Xscale  YScale  Xoffset Yoffset Size    Result?
1       1       1000    1000    24      Yes
2       2       1000    1000    24      No
1       1       264     216     24      Yes, but the spots are TINY.
10      10      264     216     24      No
10      10      100     100     24      No


This was built using the single-image tiffs under the assumption that base2
will choose the wrong offsets (0,1) for the multiimage tiff - as you can see
above genepix 6 seems to put previews first.

In future versions of base2 would it be possible to *choose* the offsets? In
2.2.2 I can only specify the same image name twice - once in the red box and
once in the green.

I'll put images somewhere as soon as I can so that you can see them.

Chad Matsalla
National Research Council of Canada




[1]
java.lang.ClassCastException: [[B
at 
com.sun.media.jai.opimage.BandMergeOpImage.shortLoop(BandMergeOpImage.java:3
41)
at 
com.sun.media.jai.opimage.BandMergeOpImage.computeRect(BandMergeOpImage.java
:199)
at javax.media.jai.PointOpImage.computeTile(PointOpImage.java:977)
at 
com.sun.media.jai.util.SunTileScheduler.scheduleTile(SunTileScheduler.java:9
12)
at javax.media.jai.OpImage.getTile(OpImage.java:1139)
at com.sun.media.jai.opimage.CropOpImage.getTile(CropOpImage.java:133)
at javax.media.jai.PlanarImage.getData(PlanarImage.java:2098)
at javax.media.jai.StatisticsOpImage.getProperty(StatisticsOpImage.java:300)
at 
com.sun.media.jai.opimage.ExtremaOpImage.getProperty(ExtremaOpImage.java:108
)
at javax.media.jai.RenderedOp$1.getProperty(RenderedOp.java:1819)
at 
javax.media.jai.PropertyEnvironment.getProperty(PropertyEnvironment.java:207
)
at 
javax.media.jai.PropertySourceImpl.getProperty(PropertySourceImpl.java:285)
at 
javax.media.jai.WritablePropertySourceImpl.getProperty(WritablePropertySourc
eImpl.java:138)
at javax.media.jai.RenderedOp.getProperty(RenderedOp.java:1993)
at net.sf.basedb.core.SpotImages.rescaleColors(SpotImages.java:805)
at net.sf.basedb.core.SpotImages.createSpotImages(SpotImages.java:499)
at net.sf.basedb.plugins.SpotImageCreator.run(SpotImageCreator.java:148)
at 
net.sf.basedb.core.PluginExecutionRequest.invoke(PluginExecutionRequest.java
:88)
at 
net.sf.basedb.core.InternalJobQueue$JobRunner.run(InternalJobQueue.java:420)
at java.lang.Thread.run(Thread.java:595)




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