Chad Matsalla wrote: > > > I'm going to analyze this array and try to put it into base as single-image > tiffs but I need to add the Yeast reporters from the galfile. Whew!
Actually, all you need is the X and Y coordinates from the raw data file. You don't need the reporters or anything else. > Note that this spotfinding process (with single image tiffs) doesn't ALWAYS > fail. I built this table to show success: > Xscale YScale Xoffset Yoffset Size Result? > 1 1 1000 1000 24 Yes > 2 2 1000 1000 24 No > 1 1 264 216 24 Yes, but the spots are TINY. > 10 10 264 216 24 No > 10 10 100 100 24 No Only one set of values are correct and if it fails with those it doesn't really matter if it works with other values. The scale and offset values can be found in the genepix file headers. The spot size has to be guessed. On our test image we use 32. > This was built using the single-image tiffs under the assumption that base2 > will choose the wrong offsets (0,1) for the multiimage tiff - as you can see > above genepix 6 seems to put previews first. > > In future versions of base2 would it be possible to *choose* the offsets? In > 2.2.2 I can only specify the same image name twice - once in the red box and > once in the green. I don't know. Is there some expert on TIFF images and possibly also the JAI toolkit out there who can help us with the problem with spot images? I am afraid the we don't have much time to spend on this issue as long as our own images are working. > I'll put images somewhere as soon as I can so that you can see them. Ok, I'll try them. I'll need the images and the raw data file. /Nicklas ------------------------------------------------------------------------- Take Surveys. Earn Cash. Influence the Future of IT Join SourceForge.net's Techsay panel and you'll get the chance to share your opinions on IT & business topics through brief surveys-and earn cash http://www.techsay.com/default.php?page=join.php&p=sourceforge&CID=DEVDEV _______________________________________________ The BASE general discussion mailing list basedb-users@lists.sourceforge.net unsubscribe: send a mail with subject "unsubscribe" to [EMAIL PROTECTED]
