Nicklas Nordborg wrote:
> Dominic Oyeniran wrote:
>> Hi Micha,
>>
>> Micha Bayer wrote:
>>> Hi Dominic,
>>>
>>> We are trying to export our first experiment for real so we can submit
>>> it to ArrayExpress, and initially it looked like everything had worked
>>> okay. But now we have found an error with the annotation that I wanted
>>> to ask you about. 
>>> Our experiment is a two-colour one, and we have a factor which can take
>>> the value of mutant or wildtype. Both  mutant and wildtype sample is
>>> applied to the hybridization (one per channel) and we then want the
>>> RawBioAssay item to inherit its annotations. The only way we could find
>>> to do that was to inherit from both the samples, but then only one of
>>> them is exported into the tab2mage file, which presumably is not good
>>> enough for ArrayExpress.
>> This is becuase BASE chooses the first of the inherited annotations 
>> values that it finds for sample, assigns and displays this as the factor 
>> value for the experiment.
> 
> BASE doesn't choose one of them. All inherited annotation values are 
> always displayed.
> 


BASE actually chooses one of them, at least as shown on the GUI here. 
It's not the exprimental factors section of the GUI parse that's 
relevant here, based on my understanding, but the Raw Bio assays section 
listing each raw bio assay included in the experiment. Since 
experimental factor values/annotation values has to be propagated 
through the Raw Bio assays.

For each Raw Bio assay in an experiment,  you can only see or get one of 
the inherited values having inherited annotations from two different 
Sample items  each from a line in the two channel hyb.

However, the inherited values are displayed as separate annotations for 
the raw bio assays on the 'Inherited annotations and protocol parameters 
section' of the view page.

If this is not the case then I must have a wrong understanding of how 
factor values annotation are obtained or handled in BASE.

¬Dominic




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