Hi,
yeast one hybrid� will help in your case if you have no
idea which gene will be the target gene.
If you have some idea on
the� target� gene,� Both� EMSA� John mentioned is
a good choice.
Good Luck!
Lichun
> I
haven't tested the procedure myself, but a paper in 2006 in BMC
>
Bioinformatics described a "machine learning approach." See Yan
et al.
> 7:262. Good luck!
>
> -----Original
Message-----
>
From: [EMAIL PROTECTED] on
behalf of Donnie Darko
> Sent: Sun 1/6/2008 9:57 AM
>
To: [email protected]
> Cc:
> Subject: [BiO BB]
Newbie question.[Scanned]
>
>
>
> Hi
folks,
>
> Suppose I have a novel gene whose product is
a DNA binding protein, how
> would I go about locating potential
binding sites where this protein
> could
> bind? I'm only
interested in finding sites for this specific protein and
> not
> a general scan of tfbs.
>
> I've extracted
upstream sequences of genes that I'm interested in but I
> can't
think of a way forward.
>
> Cheers and belated happy new
year :-P
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--
[EMAIL PROTECTED]
Institute of Biochemistry and
Cell Biology,
Shanghai Institutes for Biological Sciences,
Chinese Academy of Sciences
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