Can you remove me from your email list thanks
-----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Rebekah Rogers Sent: Friday, February 08, 2008 8:57 PM To: [email protected] Subject: [BiO BB] Inconsistent Blast Results Hi: I'm currently running blast 2.2.14 locally on my mac. I've noticed that the printout from a blastn run at an E cutoff of 10^-10 reads differently than a blast run at an E cutoff of 10^-7 when hits worse than 10^-10 are ignored. Suddenly at 10^-7 new hits with evals of 10^-11 appear that weren't there before and even the relative strength of different hits can change. I'm not certain I understand why this is true and it has a huge impact on my results. I know that the Eval is dependent on certain constants taken from the compared sequences, but I don't understand how this could possibly change when I'm using the exact same input file and database. Does anyone have an explanation? -Rebekah _______________________________________________ BBB mailing list [email protected] http://www.bioinformatics.org/mailman/listinfo/bbb _______________________________________________ BBB mailing list [email protected] http://www.bioinformatics.org/mailman/listinfo/bbb
