Please note that Apocom Genomics provides the GrailEXP software which incorporates BLAST and specific exon, Cpg island ID functions (as well as many other features). Anyone interested in learning more, should e-mail me back.
Robin Zimmer -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Rebekah Rogers Sent: Friday, February 08, 2008 8:57 PM To: [email protected] Subject: [BiO BB] Inconsistent Blast Results Hi: I'm currently running blast 2.2.14 locally on my mac. I've noticed that the printout from a blastn run at an E cutoff of 10^-10 reads differently than a blast run at an E cutoff of 10^-7 when hits worse than 10^-10 are ignored. Suddenly at 10^-7 new hits with evals of 10^-11 appear that weren't there before and even the relative strength of different hits can change. I'm not certain I understand why this is true and it has a huge impact on my results. I know that the Eval is dependent on certain constants taken from the compared sequences, but I don't understand how this could possibly change when I'm using the exact same input file and database. Does anyone have an explanation? -Rebekah _______________________________________________ BBB mailing list [email protected] http://www.bioinformatics.org/mailman/listinfo/bbb _______________________________________________ BBB mailing list [email protected] http://www.bioinformatics.org/mailman/listinfo/bbb
