Thanks Ray, Yepp, i think I need to be more specific. I am trying to design a web server which can upload a user input file which contains number of protien sequences. Then I need to blast this file to get blast results directly as an output file. But as m new to perl, I seriously dont have an idea how to proceed furthur. I have to use perl and cgi... Yepp basic URL in use to get results is *NCBI Blast.*
So layout is something like this : BOX -----> Upload File BLAST----> new button. On clicking this, I shud get the results, may be as an output file. Hope you can help me with some hints for code or smthing like that. Thanks and Regards, Jyo On Wed, Oct 7, 2009 at 3:59 AM, Raymond Wan <r....@aist.go.jp> wrote: > > Hi Jyoti, > > > Jyoti wrote: > >> How to upload any file and get results for that uploaded file through a >> redirected URL, in perl/cgi >> I mean to ask, for example, I upload a file which contains protein >> sequences. Now I want blast results for that uploaded file in my server. >> Can anyone help me with few lines of Code, so that I can upload any file >> with any number of sequences and get the results as well when I click on >> Blast Results button. >> Specific site from where I shud get results is : ( >> http://blast.ncbi.nlm.nih.gov/Blast.cgi). Can see the homepage!!! >> Anyone can also explain me in general terms.... >> Just help me to link a URL when I click a button OR directly get the >> results >> on my page ( as same as that URL). >> > > > I'm afraid you'll have to describe your problem a bit better. You want to > upload a file to NCBI (i.e., not your server), let it do some kind of > processing, and then show its results in a web page? > > Most of what you're asking is related to NCBI and Blast and NCBI has > provided an API to do things like this (I *think* -- I haven't used their > API in a long time). For example, go to here: > > http://www.ncbi.nlm.nih.gov/entrez/query/static/eutils_help.html > > And the first paragraph says, > > "Entrez Programming Utilities are tools that provide access to Entrez data > outside of the regular web query interface and may be helpful for retrieving > search results for future use in another environment." > > There's a lot of information there and you might want to go through it > first. I'm not sure if there are other API on their web site; you'll have > to check. > > Once that's done, your results will be returned from their server and > having it show up on your server (presuming you have it set up already) > should be easy. > > And as Dave said yesterday, most people on this list aren't > bioinformaticians so you should try to describe your problem in more general > terms. > > Ray > >
