Hi Jyoti,
Jyoti wrote:
Yepp, i think I need to be more specific. I am trying to design a web server which can upload a user input file which contains number of protien sequences. Then I need to blast this file to get blast results directly as an output file. But as m new to perl, I seriously dont have an idea how to proceed furthur. I have to use perl and cgi... Yepp basic URL in use to get results is *NCBI Blast.* So layout is something like this : BOX -----> Upload File BLAST----> new button. On clicking this, I shud get the results, may be as an output file. Hope you can help me with some hints for code or smthing like that.
Well, depending on what you have done so far (you haven't said how much you've accomplished until now), you will need to read up on how to set up a web server, call NCBI remotely or run blast locally, design HTML web pages. Check out the documentation for your web server, the NCBI help pages, or HTML "how-to" pages on the Internet. All of this is somewhat unrelated to Perl (IMHO).
By "being specific", I meant to say what you are trying to do with Perl...perhaps with an indication (i.e., Perl source code) of what you've already tried.
Did you consider Dave's suggestion about using netblast (which I honestly have never used)?
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