On 02/06/2017 10:13 AM, Raymond Cavalcante wrote:
Oops, I got so caught up in the setting up the code I forgot to say what the 
error was:

Error in match.arg(pruning.mode) :
  'arg' should be one of “error”, “coarse”, “fine”, “tidy”

you have a mixture of 'release' (even-numbered y in x.y.z) and 'devel' (odd-numbered y in x.y.z) Bioconductor packages, which is a recipe for disaster. (Well, disaster might be overstating it a bit...). Use BiocInstaller::biocValid() to sort this out (use BiocInstaller::biocLite() to manage your packages) and report on whether this solves your problem.

Martin


Thanks again,
Raymond

On Feb 6, 2017, at 10:10 AM, Raymond Cavalcante <rcava...@umich.edu> wrote:

Hello,

Perhaps this is related to problems in GenomeInfoDb::Seqinfo() and NCBI server 
file structure changes, but rtracklayer::import() is failing when I specify a 
genome.

See below for example code.

Thanks,
Raymond Cavalcante

library(annotatr)

# These fail
file = system.file('extdata', 'test_BED3.bed', package = 'annotatr')
r = annotatr::read_regions(con = file, format = 'bed', genome ='hg19')
gr = rtracklayer::import(con = file, format = 'bed', genome='hg19')

# These succeed
file = system.file('extdata', 'test_BED3.bed', package = 'annotatr')
r = annotatr::read_regions(con = file, format = 'bed')
gr = rtracklayer::import(con = file, format = 'bed')

My devtools::session_info() is:

Session info -------------------------------------------------------------------
setting  value
version  R version 3.3.2 (2016-10-31)
system   x86_64, darwin16.1.0
ui       unknown
language (EN)
collate  en_US.UTF-8
tz       America/Detroit
date     2017-02-06

Packages -----------------------------------------------------------------------
package                             * version  date
AnnotationDbi                         1.36.1   2017-01-27
AnnotationHub                         2.6.4    2017-01-27
annotatr                            * 1.1.7    2017-01-27
assertthat                            0.1      2013-12-06
Biobase                               2.34.0   2016-11-16
BiocGenerics                          0.20.0   2016-11-16
BiocInstaller                         1.24.0   2016-11-16
BiocParallel                          1.8.1    2016-11-16
biomaRt                               2.30.0   2016-11-16
Biostrings                            2.42.1   2017-01-27
bitops                                1.0-6    2013-08-17
BSgenome                              1.42.0   2016-11-16
colorspace                            1.3-1    2016-11-18
DBI                                   0.5-1    2016-09-10
devtools                              1.12.0   2016-12-05
digest                                0.6.10   2016-08-02
dplyr                                 0.5.0    2016-06-24
GenomeInfoDb                          1.11.7   2017-02-04
GenomicAlignments                     1.10.0   2016-11-16
GenomicFeatures                       1.26.2   2017-01-27
GenomicRanges                         1.26.2   2017-01-27
ggplot2                               2.2.1    2016-12-30
gtable                                0.2.0    2016-02-26
htmltools                             0.3.5    2016-03-21
httpuv                                1.3.3    2015-08-04
httr                                  1.2.1    2016-07-03
interactiveDisplayBase                1.12.0   2016-11-16
IRanges                               2.8.1    2017-01-27
lattice                               0.20-34  2016-09-06
lazyeval                              0.2.0    2016-06-12
magrittr                              1.5      2014-11-22
Matrix                                1.2-8    2017-01-20
memoise                               1.0.0    2016-01-29
mime                                  0.5      2016-07-07
munsell                               0.4.3    2016-02-13
org.Dm.eg.db                          3.4.0    2016-11-16
org.Hs.eg.db                          3.4.0    2016-11-16
org.Mm.eg.db                          3.4.0    2016-11-16
org.Rn.eg.db                          3.4.0    2016-11-16
plyr                                  1.8.4    2016-06-08
R6                                    2.2.0    2016-10-05
Rcpp                                  0.12.9   2017-01-14
RCurl                                 1.95-4.8 2016-03-01
readr                                 1.0.0    2016-08-03
regioneR                              1.6.0    2017-01-27
reshape2                              1.4.2    2016-10-22
Rsamtools                             1.26.1   2016-11-16
RSQLite                               1.1-2    2017-01-08
rtracklayer                           1.34.1   2016-11-16
S4Vectors                             0.12.1   2017-01-26
scales                                0.4.1    2016-11-09
shiny                                 0.14.2   2016-11-01
stringi                               1.1.2    2016-10-01
stringr                               1.1.0    2016-08-19
SummarizedExperiment                  1.4.0    2016-11-16
tibble                                1.2      2016-08-26
TxDb.Dmelanogaster.UCSC.dm3.ensGene   3.2.2    2016-11-16
TxDb.Dmelanogaster.UCSC.dm6.ensGene   3.3.0    2016-11-16
TxDb.Hsapiens.UCSC.hg19.knownGene     3.2.2    2016-11-16
TxDb.Hsapiens.UCSC.hg38.knownGene     3.4.0    2016-11-16
TxDb.Mmusculus.UCSC.mm10.knownGene    3.4.0    2016-11-16
TxDb.Mmusculus.UCSC.mm9.knownGene     3.2.2    2016-11-16
TxDb.Rnorvegicus.UCSC.rn4.ensGene     3.2.2    2016-11-16
TxDb.Rnorvegicus.UCSC.rn5.refGene     3.4.0    2016-11-16
TxDb.Rnorvegicus.UCSC.rn6.refGene     3.4.0    2016-11-16
withr                                 1.0.2    2016-06-20
XML                                   3.98-1.5 2016-11-10
xtable                                1.8-2    2016-02-05
XVector                               0.14.0   2016-11-16
yaml                                  2.1.14   2016-11-12
zlibbioc                              1.20.0   2016-11-16

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