Ola Spjuth wrote:
Hi,
I am investigating the usefulness of BioJava as a backend for sequence
management in Bioclipse (www.bioclipse.net). As a total newbie to
Biojava, I have read the tutorial, BIA examples, glanced at the API,
read my first FASTA-sequence and have come up with a few questions:
1) Is it possible to search the Biojava-l archives without having to
manually browse by month?
2) Is there a wrapper for SequenceIO.fileToBiojava(..) that
automatically detects file formats or is it necessary to distinguish
sequence formats externally, i.e. with different file-extensions? If so,
does anyone know of a complete list of file-extensions that could be
mapped to a format?
There is a deprecated piece of code available, that quite many people
actualy use
in their code still. Even though it might not be the greatest thing to
try to auto
guess file format, its the desireable thing to do in many cases.
If i just look at people in my lab, they want to open the file, they
dont want to keep
track of what file format that particular sequence was in, and so on.
So, even if file format guessing is bad, people are going to write it,
and imho its
better to have one centralised good, known to work file guesser, then
several
different implementations that differ in each persons own application.
So, my suggestion is to start with using the deprecated version thats in
biojava, if
it gets removed you can easily just copy that small part of the code
into your own
application, or as an external little jarfile.
3) How robust are the I/O-classes for different formats? The
test-library provided is rather short in my opinion and my first test
broke since there was a space in the wrong position...
4) What are the capabilities for multiple sequence alignment in Biojava?
Is it limited to parse results into Biojava objects (as in BIA) or does
it contain any stable MSA-implementations? Due to BioJavas size it is
not easy to get an overview of the current capabilities and the standard
of different parts.
There is some support for multiple alignments in biojava. The Alignment
interface
and implementations happily handle multiple alignments. And you can
choose how
to interpret it, either as SymbolList over a crossproduct alphabet, or
as individual
sequences accessable by some label.
There is a basic framework for handling multiple alignment formats in
the biojava
org.biojava.bio.seq.io package. It currently only implements two
formats, FASTA
and MSF. Most programs seem to be able to generate multiple alignment
output
into either FASTA or MSF format so you should be able to get the results
into
biojava.
5) As a novice, has anyone implemented BLAST or CLUSTALW in Java? Any
public web-services running for this?
I have been told by greater deities that implementing BLAST in java is
hard, because
the blast algorithm makes heavy use of low level data structures,
pointers ? and similar
things that are very hard to implement and controll in java. So the
resulting implementation
would most likely run pretty darn slow, and not do what you want.
Depending on what you want to do with BLAST, the biojava SSAHA
implementation
might be something you can use instead ( it works pretty ok on quite
conserved sequences,
but its not realy suited for more divergent sequences )
When it comes to webservices i just know of a few things, i have not
used any of these
to an large extent, so i cant comment on how well they work for large
sequences, big
jobs and so on.
http://www.ebi.ac.uk/Tools/webservices/services.html
http://xml.ddbj.nig.ac.jp/wsdl/index.jsp
Sadly they all use their own data encoding and service invocation setup,
so its pretty darn
annoying to use.
6) Is there some example-code on how to use DAS (as a client)?
7) How can I submit an RFE?
Sorry for so many questions in one post; I have a lot of catching up to
do and was hoping for some guidance. Some answers have probably already
been answered in earlier posts but I have not been able to search the
archives.
Cheers,
.../Ola
_______________________________________________
Biojava-l mailing list - Biojava-l@biojava.org
http://biojava.org/mailman/listinfo/biojava-l
_______________________________________________
Biojava-l mailing list - Biojava-l@biojava.org
http://biojava.org/mailman/listinfo/biojava-l
