Dear Anant Jain,

Sorry for this misunderstanding. There is no substitution matrix "PROTEIN_TERM". The constructor of the BioJava SubstitutionMatrix class requires the following arguments: FiniteAlphabet alpha and File matrixFile. As the alphabet you should use the alphabet "PROTEIN_TERM" for matrixes of protein sequences like BLOSUM etc. In the BioJava repository there is a newer version of this class that provides a function to parse a matrix without explicitely providing an alphabet (it guesses the alphabet of the matrix). I hope this helps.

Cheers
Andreas Dräger

Anant Jain wrote:
Thank You Sir,
                       I  have searched through ftp/...blast/matrices,
but did not got any file substitution matrix like PROTEIN_TERM,  if u
have can u send me it OR tell me link from where i can download the it

Thank You
Anant jain
DYPBI
Pune, India.


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