Hello all. I am struggling to get defracting crystals with a protein DNA complex. The crystals are plentiful but they do not diffract. I am going back to the grind stone and relookong at my DNA sequence.Is there any wisdom you could give me with regards to what works best with DNA in crystals?From my reading it seems if the length is a multiple of 7 (for B DNA) and blunt ended, it will stretch over the length of the crystal and improve crystalisability. But if you want crystals that diffract better, you will need to play with length and even making it only one base longer or shorter can make a difference, even changing the morphology of the crystal? Longer is better than shorter, and overhangs are good for improving diffraction? Presumably because they stabilize contacts? It is expensive to synthesize a while bunch of sequences so I need to be strategic in my choice. Would appreciate any advice.Thank youCareina.
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