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Hallo Nada, there are probably many people who made good experience with the presence of imidazole for crystallisation as there are many with bad experience. However, from what you were writing it sounds as though you want to crystallise the protein straight from the Ni-column. From my experience - and I dare say most people will agree - it is a good idea to have more than one or two purification steps. And a side effect of e.g. a size exclusion column would be that you can conveniently get rid of the imidazole, and most protein crystallographers I met would probably do so. If, however, your protein likes imidazole or even needs it for solubility, then obviously you keep the concentration throughout your purification. Hope this helps, Tim -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A On Wed, 14 Sep 2005, Nada wrote: > Hello. > > Please I would like to know if someone has had some experience with > crystallization of protein in solution containing also 1M imidazole. Is it > necessary to remove it from solution after elution of protein from > Ni-column? > > Thank you > > Nada Pavlendova > > IMB, Bratislava, Slovakia > >
