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-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Hello Mario, it is not an answer to your question, but with 3degreess mosaicity and 0.1 degrees slices, you may want to try XDS, too, to integrate your data ( I think it is usually a good idea to use more than one integration program for comparison). Tim - -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A On Thu, 15 Sep 2005, Mario Sanches wrote: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > I am integrating two datasets using mosflm and in both cases the program seems > to underestimate the mosaicity. I tried to use the option POSTREF MOSADD X, as > mentioned on the manual, but the mosaicities either keep refining to a lower > value or diverge to an excessively high number. > > Those are derivatives datasets, both in C2. The first one has a normal > mosaicity > of about 0.7. The second has a very high mosaicity of about 3.0 (or so I > think) > and was collected in fine slices of 0.1 degree. In both cases I was able to > integrate fixing the mosaicity, but with a few problems (the normal plot > doesn't > look very nice, for example). Here are my questions: > > 1. How to tell mosflm to refine the mosaicity on a value higuer than the > initially estimated one? As I told, I tried varying the POSTREF MOSADD X > without > success. The mosaicity seems to change slightly from image to image, so I > would > like to properly refine this parameter. > > 2. In the second dataset with mosaicity around 3 the program rejects about > half > of the reflexion which are to wide in phi. Is there any way to keep some of > those reflexions or those might be rejected? > > 3. In this fine sliced/high mosaic dataset the Rmerge per image (SCALA) tend > to > be higher than usual. Is that normal in this case? > > 4. How should one interpret the anomalous plot outputted by SCALA? > > If anyone want to take a look at some images I will be more than happy to > provide them. > > Sincerely, > -- > Mario Sanches, Ph.D. > Brazilian Synchrotron Light Laboratory > Structural Molecular Biology Center > Crystallography and Spectroscopy of Biomolecules > > > -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.1 (GNU/Linux) iD8DBQFDKmqXUxlJ7aRr7hoRAhshAKDmlnZEBP4UcY0QRFmGNasWth9zHwCg2RTk Nsh261mdX7Za21G1V0RW8es= =86Hh -----END PGP SIGNATURE-----
