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Hi,
you can use foldhunter or helixhunter
http://ncmi.bcm.tmc.edu/homes/stevel/EMAN/doc/progs/foldhunter.html
I would strongly recommend that you don't take the scale of the EM data
for granted change the size of the EM model by +/- 10% of the diameter
in steps of e.g. 1% and sue that as a template. You can change the scale
using ezd maps and changing the cell sizes - just as a little hint.
Juergen
Satheesh Kumar Palani Nathan wrote:
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Dear members,
I am trying to use cryo electron microscope images ( of about 9 A
resolution) to build a search model for molecular replacement trials
(against 2.7 A x-ray diffraction data). As, I don’t have any promising
structural homolog for this protein I need to start the model building
from the scratch.
Has any one tried this approach before? Is there any automated
software to build or align secondary structural elements inside an
E-M. Image? Please share your experience and suggestions in this regard.
Thanks in advance
Satheesh
Dr. P.Satheesh Kumar
Postdoctoral Research Associate
Department of Biochemistry and Biophysics
Texas A&M University, 2128 TAMU
College Station, TX - 77843-2128
USA.
Ph. 1-979-862-7639
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Jürgen Bosch
University of Washington
STRUCTURAL GENOMICS OF PATHOGENIC PROTOZOA
Health Science Building
Dept. of Biochemistry, K-418
Box 357742
1705 NE Pacific Street
Seattle, WA 98195-7742
Phone: 206-616-4542
FAX: 206-685-7002
Web: www.sgpp.org
